期刊名：Methods and Protocols

期刊主页：https://www.mdpi.com/journal/mps

本期编辑荐读为您精选2024和2025年发表于 Methods and Protocols 期刊的十篇高浏览文章。希望能为相关领域学者提供新的思路和参考，欢迎阅读。

1. Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol

蔗糖素补充对超重或肥胖受试者血糖反应、食欲和肠道微生物群的影响：一项随机交叉研究方案

Zeniff Reyes-López, Viridiana Olvera-Hernández, Meztli Ramos-García, José D. Méndez, Crystell G. Guzmán-Priego, Miriam C. Martínez-López, Carlos García-Vázquez, Carina S. Alvarez-Villagomez, Isela E. Juárez-Rojop, Juan C. Díaz-Zagoya and Jorge L. Ble-Castillo

https://www.mdpi.com/2409-9279/7/5/80

2. The Allium cepa Model: A Review of Its Application as a Cytogenetic Tool for Evaluating the Biosafety Potential of Plant Extracts

洋葱（Allium cepa）模型：其作为细胞遗传学工具用于评估植物提取物生物安全性的应用综述

Daniela Nicuta, Luminita Grosu, Oana-Irina Patriciu, Roxana-Elena Voicu and Irina-Claudia Alexa

https://www.mdpi.com/2409-9279/8/4/88

3. A Review of Histological Techniques for Differentiating Human Bone from Animal Bone

用于区分人骨与动物骨的组织学技术综述

Emanuela Stan, Camelia-Oana Muresan, Ecaterina Daescu, Raluca Dumache, Veronica Ciocan, Stefania Ungureanu, Dan Costachescu and Alexandra Enache

https://www.mdpi.com/2409-9279/7/4/51

4. Simple Determination of Affinity Constants of Antibodies by Competitive Immunoassays

通过竞争性免疫分析简易测定抗体亲和常数

Janina Fischer, Jan Ole Kaufmann and Michael G. Weller

https://www.mdpi.com/2409-9279/7/3/49

5. In Vitro Methods for Assessing the Antibacterial and Antibiofilm Properties of Essential Oils as Potential Root Canal Irrigants—A Simplified Description of the Technical Steps

体外评估精油作为潜在根管冲洗剂的抗菌和抗生物膜特性的方法—技术步骤的简化说明

Jihad Diouchi, Jelena Marinkovi´c, Milica Nemoda, Lhoussaine El Rhaffari, Babacar Toure and Sonia Ghoul

https://www.mdpi.com/2409-9279/7/4/50

6. Development and Validation of Micro-Azocasein Assay for Quantifying Bromelain

用于菠萝蛋白酶定量的微量偶氮酪蛋白测定法的开发与验证

Krishna Pillai, Javed Akhter, Ahmed H. Mekkawy, Sarah J. Valle and David L. Morris

https://www.mdpi.com/2409-9279/7/2/25

7. Mechanisms of Resistance to CAR T-Cells and How to Overcome Them

对 CAR T 细胞的抗药机制及如何克服这些抗药性

Luca Legato, Matteo Bisio, Filippo Fasano, Corrado Benevolo Savelli, Carolina Secreto, Chiara Maria Dellacasa, Barbara Botto, Alessandro Busca, Marco Cerrano, Roberto Freilone and Mattia Novo

https://www.mdpi.com/2409-9279/8/5/108

8. Optimization of Nile Tilapia Artificial Breeding Using Human Chorionic Gonadotropin (hCG) Hormone

利用人绒毛膜促性腺激素（hCG）优化尼罗罗非鱼的人工繁殖

Golam Rbbani, Prabhugouda Siriyappagouder, Riaz Murshed, Rajesh Joshi, Artem Nedoluzhko, Jorge Galindo-Villegas and Jorge M. O. Fernandes

https://www.mdpi.com/2409-9279/8/3/57

9. Comparative Analysis of Methods for Assessing On-Target Gene Editing Efficiencies

靶向基因编辑效率评估方法的比较分析

Bing Yao, Qiangbing Yang, Manuel A. F. V. Goncalves, Raymond Schiffelers, Joost P. G. Sluijter

and Zhiyong Lei

https://www.mdpi.com/2409-9279/8/2/23

10. Biodegradable Microneedle for Enhanced Transdermal Drug Delivery: Trends and Techniques

用于增强经皮给药的可生物降解微针：趋势与技术

Renuka Khatik, Jatin Kumar Sahu, Shuvadip Bhowmik, Isha Rai, Madhu Kumari and Monika Dwivedi

https://www.mdpi.com/2409-9279/8/6/134

Methods and Protocols期刊介绍

主编：Prof. Dr. Fernando Albericio

1. School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa

2. Department of Organic Chemistry, University of Barcelona, Barcelona, Spain

Methods and Protocols是一个国际化、经同行评审的开放获取期刊。我们的目标是建立和描述生命科学、化学和生物医学等领域的多学科期刊。期刊鼓励科学家在现有技术的基础上发表新的协议，以及前沿方法的重大改进和发展。目前期刊现已被ESCI, Scopus, PubMed, PMC等数据库收录。

欢迎订阅Methods and Protocols期刊：https://www.mdpi.com/journal/mps