期刊名：Methods and Protocols

期刊主页：https://www.mdpi.com/journal/mps

本期编辑荐读为您精选6篇2025年封面文章。希望能为相关领域学者提供新的思路和参考，欢迎阅读。

1. Simplified Protocol for the Purification of Native Cas Nucleases for DNA-Free Genome Editing

用于无DNA基因组编辑的天然Cas核酸酶纯化简化方案

Margherita D’Amico, Flavia Angela Maria Maggiolini, Lucia Rosaria Forleo, Maria Francesca Cardone, Riccardo Velasco, Teodora Basile and Carlo Bergamini

https://www.mdpi.com/2409-9279/8/1/16

2. Mapping Gene Expression in Whole Larval Brains of Bicyclus anynana Butterflies

非洲灰蝶（Bicyclus anynana）幼虫全脑基因表达图谱构建

Tirtha Das Banerjee, Linwan Zhang and Antonia Monteiro

https://www.mdpi.com/2409-9279/8/2/31

3. The Validation of Antibodies Suitable for Flow Cytometric Analysis and Immunopeptidomics of Peptide–MHC Complexes in the Outbred Swiss Albino Mouse Strain

适用于外繁瑞士白化小鼠品系的肽–MHC复合物流式细胞术分析和免疫肽组学研究的抗体验证

Shanzou Chung, Isambard G. Knox-Johnson, Sarah E. Gazzard, Runqiu Song, Ngoc H. Le,Luise A. Cullen-McEwen, John F. Bertram, Anthony W. Purcell and Asolina Braun

https://www.mdpi.com/2073-4468/14/3/72

4. K562 Chronic Myeloid Leukemia Cells as a Dual β3-Expressing Functional Cell Line Model to Investigate the Effects of Combined αIIbβ3 and αvβ3 Antagonism

K562慢性髓性白血病细胞作为双β3表达的功能性细胞系模型，用于研究联合拮抗αIIbβ3和αvβ3的效果

Amal A. Elsharif, Laurence H. Patterson, Steven D. Shnyder and Helen M. Sheldrake

https://www.mdpi.com/2409-9279/8/4/73

5. Mechanisms of Resistance to CAR T-Cells and How to Overcome Them

对 CAR T 细胞的抗药机制及如何克服这些抗药性

Luca Legato, Matteo Bisio, Filippo Fasano, Corrado Benevolo Savelli, Carolina Secreto, Chiara Maria Dellacasa, Barbara Botto, Alessandro Busca, Marco Cerrano, Roberto Freilone and Mattia Novo

https://www.mdpi.com/2409-9279/8/5/108

6. Fluorescence-Based Detection of KRAS Mutations in Genomic DNA Using Magnetic Bead-Coupled LDR Assay

利用磁珠偶联LDR方法在基因组DNA中进行KRAS突变的荧光检测

Chika Morimoto and Masahiko Hashimoto

https://www.mdpi.com/2409-9279/8/6/142

Methods and Protocols期刊介绍

主编：Prof. Dr. Fernando Albericio

1. School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa

2. Department of Organic Chemistry, University of Barcelona, Barcelona, Spain

Methods and Protocols是一个国际化、经同行评审的开放获取期刊。我们的目标是建立和描述生命科学、化学和生物医学等领域的多学科期刊。期刊鼓励科学家在现有技术的基础上发表新的协议，以及前沿方法的重大改进和发展。目前期刊现已被ESCI, Scopus, PubMed, PMC等数据库收录。

欢迎订阅Methods and Protocols期刊：https://www.mdpi.com/journal/mps