在这项研究中，该课题组研究人员开发了一种根表面外胞体ROS的实时成像方法。不同的信号，包括生长素、细胞外三磷酸腺苷和快速碱化因子1肽，诱导胞质钙瞬变和外胞体ROS爆发。遗传和光遗传学方法发现，通过激活还原性烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶RBOHC和RBOHF，钙瞬态对ROS爆发是必要和充分的。外胞体ROS爆发不是必需的，但它们确实限制了重力诱导的根弯曲。根弯曲被拉伸激活的钙通道MCA1感知，导致NADPH氧化酶激活。由此产生的活性氧使细胞壁变硬，以促进土壤渗透。它们提供了一种平衡组织灵活性和刚度以导航土壤的方法。
据介绍，活性氧（Reactive oxygen species， ROS）参与了植物的多种信号传递过程，但其潜在的机制和作用仍然是一个谜。
附：英文原文
Title: Calcium-triggered apoplastic ROS bursts balance gravity and mechanical signals for soil navigation
Author: Ivan Kulich, Dmitrii Vladimirtsev, Marek Randuch, Shiqiang Gao, Matteo Citterico, Kai R. Konrad, Georg Nagel, Michael Wrzaczek, Léa Cascaro, Pauline Vinet, Pauline Durand, Atef Asnacios, Lokesh Verma, Malcolm J. Bennett, Bipin K. Pandey, Jií Friml
Issue&Volume: 2026-04-16
Abstract: Reactive oxygen species (ROS) have been implicated in multiple signaling processes in plants, but the underlying mechanisms and roles remain enigmatic. In this study, we developed a method of live imaging of apoplastic ROS at the root surface. Distinct signals, including auxin, extracellular adenosine triphosphate, and rapid alkalinization factor 1 peptide, induce cytosolic calcium transients and apoplastic ROS bursts. Genetic and optogenetic manipulations of Arabidopsis identified calcium transients as necessary and sufficient for ROS bursts through activation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases RBOHC and RBOHF. Apoplastic ROS bursts are not required, but they do limit gravity-induced root bending. Root bending is sensed by the stretch-activated calcium channel MCA1, leading to NADPH oxidase activation. The resulting ROS production stiffens cell walls to facilitate soil penetration. Apoplastic ROS thus provides a means to balance tissue flexibility and stiffness to navigate soil.
DOI: adu8197
Source: https://www.science.org/doi/10.1126/science.adu8197