研究小组证明了肌动蛋白结合的formin SYFO2在根瘤菌感染中是必不可少的，在根瘤菌感染中，它在相分离和共生特异性纳米结构域中驱动肌动蛋白聚合。SYFO2还调节根瘤枝外植物菌根共生过程中形成的共生活性菌根，表明它还被招募来促进豆科植物的根瘤菌感染。为了在非豆科植物中实现固氮，小组通过稳定地将RNS主调控因子NODULE INCEPTION （NIN）引入天然非寄主番茄中，激活了内源性SYFO2。这证明了将结核菌根相关基因募集到工程结核特异性途径中的可能性。
据介绍，植物根系的定植需要大量的形态动力学重组。例如，豆科植物在根瘤共生（RNS）过程中形成的称为感染袋的肌动蛋白支架微室。
附：英文原文
Title: Nanodomain-localized formin gates symbiotic microbial entry in legume and solanaceous plants
Author: Lijin Qiao, Heng Sun, Jiping Tang, Casandra Hernández-Reyes, Beatrice Lace, Julian Knerr, Eija Schulze, Tak Lee, Jean Keller, Cyril Libourel, Jilin Yao, Feiyang Zhao, Ying Ni, Yutian Jia, Xia Xu, Guanghui Yang, Lin Zhang, Yanli Zhang, Robert Grosse, Changfu Tian, Giles E. D. Oldroyd, Pierre-Marc Delaux, Thomas Ott, Pengbo Liang
Issue&Volume: 2026-03-05
Abstract: Colonization of plant roots by symbionts requires substantial morphodynamic reorganization. Examples are actin-scaffolded microcompartments called infection pockets formed during root nodule symbiosis (RNS) by legumes. We demonstrate that the actin-binding formin SYFO2 is indispensable for rhizobial infection in Medicago truncatula, where it drives actin polymerization in phase-separated and symbiosis-specific nanodomains. SYFO2 also regulates symbiotically active arbuscules formed during mycorrhizal symbiosis in plants outside the nodulating clade, indicating that it was additionally recruited to promote rhizobial infections in legumes. As part of our aim to enable nitrogen fixation in nonlegumes, we activated endogenous SYFO2 by stably introducing the RNS master regulator NODULE INCEPTION (NIN) into the natural nonhost tomato. This demonstrates the possibility of recruiting arbuscular mycorrhizae–related genes into an engineered nodulation-specific pathway.
DOI: adx8542
Source: https://www.science.org/doi/10.1126/science.adx8542