真核RNA聚合酶机制的活细胞单分子动力学，这一成果由约翰斯·霍普金斯大学
利用活酵母的单分子跟踪，该研究团队量化了58个包含三种RNAP机制的蛋白质的动力学。与短暂的RNAPII PIC相比，RNAPI和RNAPIII预起始复合物（PICs）参与长寿命的染色质相互作用。该研究团队进一步报道了RNAPII相关因子的延伸、组蛋白修饰、C端结构域（CTD）修饰、RNA加工和终止的动力学。许多伸长因子表现出短暂而非持久的关联，表明与因子交换的动态相互作用，允许潜在的调节事件。CTD截断减少了U1 snRNP在核糖体蛋白基因中的停留时间和内含子保留，为共转录剪接提供了见解。他们的发现建立了RNAP机制动态相互作用的框架。
据介绍，真核生物的基因表达是由RNA聚合酶（RNAPI， II和III）和相关因子调控的，但它们的实时动态尚不清楚。
附：英文原文
Title: Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries
Author: Yick Hin Ling, Chloe Liang, Sixiang Wang, Carl Wu
Issue&Volume: 2026-02-05
Abstract: Eukaryotic gene expression is orchestrated by RNA polymerases (RNAPI, II, and III) and associated factors, yet their real-time dynamics remain obscure. Using single-molecule tracking in living yeast, we quantified the kinetics of 58 proteins encompassing three RNAP machineries. RNAPI and RNAPIII pre-initiation complexes (PICs) engage in long-lived chromatin interactions, contrasting with transient RNAPII PIC. We further report kinetics of RNAPII-associated factors for elongation, histone modification, C-terminal domain (CTD) modification, RNA processing, and termination. Many elongation factors show brief rather than persistent association, suggesting dynamic interactions with factor exchange, allowing a potential repertoire of regulatory events. CTD truncation reduces U1 snRNP residence time and intron retention in ribosomal protein genes, providing insights into co-transcriptional splicing. Our findings establish a framework of dynamic interactions of RNAP machineries.
DOI: ads0960
Source: https://www.science.org/doi/10.1126/science.ads0960