近日，兰州大学汪宝堆团队研究了高效氨基酸对映体分离的单手性氢键生物杂化纳米通道膜。2026年2月24日出版的《美国化学会志》发表了这项成果。

均相手性纳米通道膜在大规模对映体分离领域前景广阔，但由于结构不稳定和活性位点不均一，实现高通量下的高对映选择性仍具挑战。

研究组首次报道了一种电压驱动的原位组装策略，在聚对苯二甲酸乙二醇酯（PET）纳米通道内构建均相手性氢键生物杂化框架（HBF@PET）。通过牛血清白蛋白与1,3,6,8-四（对苯二甲酸）芘（H4TBAPy）之间的定向氢键作用，该膜继承了BSA的手性微环境，同时实现了卓越的结构稳定性。HBF@PET膜能够近乎完全拆分组氨酸对映体（ee >99%），其中d-型组氨酸优先传输，通量达到创纪录的4.52 ± 0.04 mmol m^-2 h^-1，超越了现有最先进的纳米通道系统。

机理研究表明，HBF对l-型组氨酸具有更强的结合亲和力，从而阻碍其扩散并促进d-型组氨酸的渗透。该膜进一步展示了对色氨酸和精氨酸对映体的广泛适用性。这项工作开创了在纳米通道内构建蛋白质导向氢键框架的先河，为制药领域的高效手性分离提供了一个可扩展的平台。

附：英文原文

Title: Robust Homochiral Hydrogen-Bonded Biohybrid Nanochannel Membranes for High-Efficiency Enantioseparation of Amino Acids

Author: Xinyue Chang, Yanxia Song, Liping Zhen, Ningshuang Gao, Zhiwen Zhao, Genping Meng, Jinglai Duan, Baodui Wang

Issue&Volume: February 24, 2026

Abstract: Homochiral nanochannel membranes hold promise for scalable enantiomer separation, yet achieving high enantioselectivity with robust flux remains challenging due to structural instability and heterogeneous active sites. Here, we report for the first time a voltage-driven in situ assembly strategy to construct a homochiral hydrogen-bonded biohybrid framework (HBF) within poly(ethylene terephthalate) (PET) nanochannels (HBF@PET). Leveraging directional hydrogen bonding between bovine serum albumin and 1,3,6,8-tetra (terephthalic acid) pyrene (H4TBAPy), the membrane inherits BSA’s chiral microenvironment while achieving exceptional structural stability. The HBF@PET membrane enables near-complete resolution of racemic histidine (ee >99%) with d-His preferentially transported at a record flux of 4.52 ± 0.04 mmol m–2 h–1, surpassing state-of-the-art nanochannel systems. Mechanistic studies reveal stronger binding affinity of HBF for l-His, hindering its diffusion while facilitating d-His permeation. The membrane further demonstrates broad applicability to tryptophan and arginine enantiomers. This work pioneers the development of protein-guided hydrogen-bonded frameworks within nanochannels, offering a scalable platform for high-efficiency chiral separations in pharmaceuticals.

DOI: 10.1021/jacs.5c22150

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.5c22150