近日，华东师范大学钱旭红团队报道了针对脂质氢提取的早期铁死亡检测。相关论文于2026年2月9日发表在《美国化学会志》上。

铁死亡是一种铁离子依赖性、由脂质过氧化介导的程序性细胞死亡。其早期检测对疾病诊断与及时干预至关重要。脂质氢提取是“脂质过氧化-铁死亡”级联反应的起始步骤，也是铁死亡早期检测的理想靶点。

研究组开发了首组用于检测脂质氢提取的荧光探针：红色荧光探针LHA585、深红色探针LHA675以及近红外探针LHA930。其设计关键在于使用4-苯基-3-甲基丁-2-烯基结构单元——该单元高度模拟多不饱和脂质的化学特性，能够特异性检测铁死亡过程中产生的高活性自由基。

值得注意的是，在体外氧糖剥夺/复氧模型中，LHA585产生荧光开启信号的时间比当前金标准探针约早8小时，凸显了其在铁死亡早期检测方面的优越性。LHA585/675适用于体外研究，而LHA930则能实现体内铁死亡检测。这些研究成果共同确立了LHA探针体系作为铁死亡检测的重要进展，为深入探究脂质过氧化驱动的细胞死亡机制提供了有力工具。

附：英文原文

Title: Early Ferroptosis Detection Targeting Lipid Hydrogen Abstraction

Author: Cankun Li, Cheng Yao, Ruiqi Su, Yanyan Deng, Chunchun Jiang, Yuyang Zhang, Jiamu Wei, Lili Li, Linyan Huang, Yubo Lin, Guangbo Ge, Suhua Qi, Xuhong Qian, Xiao Luo, Youjun Yang

Issue&Volume: February 9, 2026

Abstract: Ferroptosis is an Fe2+-dependent and lipid peroxidation-mediated regulated cell death. Its early detection is critical for diagnosis and timely intervention. Lipid hydrogen abstraction is the initiation step of the “lipid peroxidation-ferroptosis” cascade and an ideal target for early detection of ferroptosis. We developed the first set of fluorogenic probes for lipid H-abstraction, i.e., LHA585 for red, LHA675 for deep-red, and LHA930 for near-infrared. The key to their design is the use of 4-phenyl-3-methylbut-2-enyl, a close mimic of the polyunsaturated lipid, to specifically detect the highly oxidative radicals involved in ferroptosis. Notably, LHA585 yielded a fluorescence turn-on ca. 8 h earlier than the current gold-standard probe in an in vitro OGD/R model, highlighting its superiority for early detection of ferroptosis. While LHA585/675 is intended for in vitro studies, LHA930 was feasible for in vivo ferroptosis detection. Collectively, these findings establish LHAs as a robust advance for ferroptosis sensing, enabling in-depth mechanistic studies of lipid-peroxidation-driven cell death.

DOI: 10.1021/jacs.5c17084

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.5c17084