近日，香港中文大学（深圳）唐本忠团队研究了NIR-I和NIR-II荧光团的机械化学受体工程，实现输尿管血管正交成像引导手术。2025年12月29日出版的《美国化学会志》发表了这项成果。

在腹腔镜手术中，术中精准识别输尿管及周围血管结构对于预防医源性损伤至关重要。虽然荧光成像技术为此提供了可行方案，但传统的单通道系统缺乏对多结构进行解剖学区分的能力。

研究组提出一种通过机械化学辅助的受体工程实现的双通道近红外荧光成像策略，该策略能据此筛选出光谱正交的荧光团。由此获得的两款近红外荧光染料TQx-FTA（近红外一区，发射波长755 nm）和TPzCl-FTA（近红外二区，发射波长1122 nm）展现出高达280 nm的大斯托克斯位移、极低的光谱重叠度与优异的光稳定性。当将其制备成纳米颗粒制剂后，在白光与980 nm激发光源下均可实现高信背比且无串扰的成像效果。

在兔模型中，无论采用顺行或逆行灌注方案，该系统均能实现对输尿管及血管结构的高对比度显影。该系统可准确检测输尿管狭窄、梗阻及损伤性渗漏等病理状况，定量荧光谱线进一步证实了其解剖定位的精确性。与单通道成像相比，双通道系统展现出更卓越的结构区分能力。这一融合化学合成与成像技术的平台克服了传统荧光成像的关键局限，为高分辨率手术导航提供了具备临床转化潜力的解决方案。

附：英文原文

Title: Mechanochemical Acceptor Engineering for NIR-I and NIR-II Fluorophores Enabling Orthogonal Ureter-Vascular Image-Guided Surgery

Author: Huilin Xie, Zhen Tian, Rongyuan Zhang, Zhihui Huang, Yucheng Wang, Zonghang Liu, Ryan T. K. Kwok, Jacky W. Y. Lam, Zheng Zhao, Yuhua Huang, Ben Zhong Tang, Jianquan Zhang

Issue&Volume: December 29, 2025

Abstract: Accurate intraoperative identification of the ureters and surrounding vasculature is essential for preventing iatrogenic injuries during laparoscopic surgery. While fluorescence imaging (FLI) offers a promising solution, conventional single-channel systems lack the capacity for multistructure anatomical differentiation. Here, we present a dual-channel near-infrared (NIR) FLI strategy facilitated by mechanochemistry-assisted acceptor engineering, which allows the subsequent screening of spectrally orthogonal fluorophores. Two of the resulting NIR fluorophores, TQx-FTA (NIR-I, λem = 755 nm) and TPzCl-FTA (NIR-II, λem = 1122 nm), exhibit large Stokes shifts of up to 280 nm, minimal spectral overlap, and high photostability. When formulated into nanoparticles, they enable high signal-to-background ratios and crosstalk-free imaging under white-light and 980 nm excitation. In rabbit models, both antegrade and retrograde infusion workflows provided high-contrast visualization of the ureters and vasculature. The system accurately detected ureteral pathologies, including strictures, obstructions, and injury-induced leakage, with quantitative fluorescence profiles confirming the anatomical precision. Compared to single-channel imaging, the dual-channel system offered a superior ability for structural differentiation. This integrated chemical and imaging platform overcomes key limitations in conventional FLI and offers a clinically translatable approach for high-resolution surgical navigation.

DOI: 10.1021/jacs.5c17089

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.5c17089