通过生物信息学和合成生物学方法，该课题组从285种植物物种中鉴定了LRR-RLK-XIIs，并鉴定了一种感知冷休克蛋白（CSP）肽的受体“SCORE”。来自多个被子植物谱系的SCORE同源物表现出CSP识别多态性，表明通过关键氨基酸残基的替换，反复选择病原体识别。通过功能系统基因组学和蛋白质结构预测，课题组研究人员设计了能够检测多种植物病原体CSP肽的SCORE变异，它们揭示了植物中不同的PRR识别景观。他们的策略为改造植物免疫受体，特别是多年生作物带来了希望。
据悉，植物部署了多种模式识别受体（PRRs），它们感知微生物相关的分子模式来激活免疫反应。由于谱系特异性多样化，富亮氨酸重复受体样激酶XII亚群（LRR-RLK-XII）代表了最大的PRR家族之一。
附：英文原文
Title: Systematic discovery and engineering of synthetic immune receptors in plants
Author: Bruno Pok Man Ngou, Michele Wyler, Marc W. Schmid, Takehiro Suzuki, Markus Albert, Naoshi Dohmae, Yasuhiro Kadota, Ken Shirasu
Issue&Volume: 2025-09-04
Abstract: Plants deploy a diverse array of pattern recognition receptors (PRRs), which perceive microbe-associated molecular patterns to activate immune responses. Leucine-rich repeat receptor-like kinase subgroup XII (LRR-RLK-XII) represents one of the largest PRR families owing to lineage-specific diversification. Through bioinformatics and synthetic biology approaches, we characterized LRR-RLK-XIIs from 285 plant species and identified a receptor, “SCORE,” that perceives cold shock protein (CSP) peptides. SCORE orthologs from multiple angiosperm lineages exhibit CSP recognition polymorphisms, indicating recurrent selection for pathogen recognition through substitutions at key amino acid residues. Through functional phylogenomics and protein structure predictions, we engineered SCORE variants capable of detecting multiple phytopathogen CSP peptides, thus revealing the diverse PRR recognition landscape in plants. Our strategy holds promise for engineering plant immune receptors, particularly for perennial crops.
DOI: adx2508
Source: https://www.science.org/doi/10.1126/science.adx2508