武汉大学孙蒙祥团队近日取得一项新成果。经过不懈努力，他们研究出通过BBM-BAR1对小孢子命运重编程高效体内单倍体诱导。该研究于2025年9月3日发表于国际一流学术期刊《细胞》杂志上。

该研究团队证明了BABY BOOM（BBM）的小孢子特异性表达足以诱导烟草和水稻的小孢子细胞命运转变和体内雄激素发生，有效地绕过了胁迫处理的需要。课题组研究人员进一步发现BBM激活的雄激素生成调节因子1（BAR1）是BBM促进小孢子重编程的新型下游效应物。值得注意的是，BBM和BAR1都可以取代胁迫处理在小孢子发育重编程和触发雄激素发生中的作用。这项研究揭示了一个保守的雄激素发生调控模块，为克服长期存在的限制和实现不同作物体内高效的HI提供了一种变革性的方法。

据了解，半个多世纪以来，通过胁迫处理的小孢子培养诱导单倍体（HI）已经引起了广泛的关注，但小孢子命运转变的分子机制仍然知之甚少。

附：英文原文

Title: Reprogramming of microspore fate via BBM-BAR1 for highly efficient in vivo haploid induction

Author: Ce Shi, Zifu Zhao, Yicheng Zhong, Ying Qiao, Lai Zhang, Fan Yang, Siyuan Li, Lulu Li, Lanjie Huang, Xingchun Tang, Pan Luo, Meng-Xiang Sun

Issue&Volume: 2025-09-03

Abstract: Haploid induction (HI) through stress-treated microspore culture has gained significant attention for over half a century, yet the molecular mechanism underlying microspore fate transition for androgenesis remains poorly understood. Here, we demonstrate that microspore-specific expression of BABY BOOM (BBM) is sufficient to induce microspore cell fate transition and in vivo androgenesis in both tobacco and rice, effectively bypassing the requirement for stress treatment. We further identify BBM-activated Androgenesis Regulator 1 (BAR1) as a novel downstream effector of BBM that promotes microspore reprogramming. Remarkably, both BBM and BAR1 can replace the role of stress treatment in reprogramming microspore development and triggering androgenesis. This study reveals a conserved regulatory module governing androgenesis, providing a transformative approach to overcome long-standing limitations and enable highly efficient in vivo HI across diverse crops.

DOI: 10.1016/j.cell.2025.08.014

Source: https://www.cell.com/cell/abstract/S0092-8674(25)00928-6