四川大学林宏辉课题组近日取得一项新成果。经过不懈努力，他们的研究报道了萝卜（Raphanus sativus L.）端粒-端粒基因组组装为抽苔性状的QTL定位提供了新的见解。相关论文于2025年8月7日发表于国际顶尖学术期刊《遗传学报》杂志上。

萝卜（Raphanthem sativthem L.）是一种重要的十字花科根茎蔬菜，其抽苔受多基因调控；然而，脱根调控的遗传机制尚不清楚。利用Oxford Nanopore、PacBio和Hi-C测序技术，将品种C60213的基因组组装成一个高质量、无端粒到端粒间隙的结构，跨越9条染色体，总计472.71Mb。共鉴定出49768个蛋白编码基因，其中97.38%被功能注释。重复序列占基因组的59.72%，主要是长末端重复序列。以早、晚抽苔的F2群体为研究对象，构建了高密度遗传连锁图谱，确定了与抽苔和开花相关的7个主要数量性状位点。RNA-seq和实时荧光定量PCR分析发现，RsMIPS3基因与抽苔有关，且在抽苔过程中表达减少。值得注意的是，RsMIPS3在拟南芥中的过表达延迟了抽苔时间，证实了其在调节抽苔时间中的作用。这些发现促进了萝卜基因组的研究，并为晚抽苔品种的选育提供了有价值的靶点。

附：英文原文

Title: A telomere-to-telomere genome assembly of radish (Raphanus sativus L.) provides insights into QTL mapping of bolting traits

Author: Dawei Zhang b, Honghui Lin b

Issue&Volume: 2025/08/07

Abstract: Radish (Raphanus sativus L.) is an important cruciferous root vegetable, with bolting regulated by multiple genes; however, the genetic mechanisms underlying bolting regulation remain unclear. Here, the genome of the cultivar C60213 is assembled into a high-quality, gap-free telomere-to-telomere structure, spanning nine chromosomes and totaling 472.71 Mb, using a combination of Oxford Nanopore, PacBio, and Hi-C sequencing technologies. It identifies 49,768 protein-coding genes, 97.38% of which are functionally annotated. Repetitive sequences constitute 59.72% of the genome, primarily comprising long terminal repeats. A high-density genetic linkage map is constructed using an F2 population derived from a cross between early- and late-bolting radishes, identifying seven major quantitative trait loci associated with bolting and flowering. RNA-seq and quantitative real-time PCR analysis reveal that the RsMIPS3 gene is found to be associated with bolting, with its expression decreasing during this process. Notably, RsMIPS3 overexpression in Arabidopsis delays bolting, confirming its role in regulating bolting time. These findings advance radish genome research and provide a valuable target for breeding late-bolting varieties.

DOI: 10.1016/j.jgg.2025.07.014

Source: https://www.sciencedirect.com/science/article/abs/pii/S167385272500222X