近日，华东师范大学张诗按团队研究了频率梳声光相干编码（FACE）的超高通量单像素复场显微镜。相关论文于2025年8月11日发表在《光：科学与应用》杂志上。

单像素成像（SPI）是一种很有前途的光学成像技术，用于超越可见光谱，商用相机价格昂贵或不可用。然而，缓慢的模式投影速率和耗时的重建算法等限制阻碍了其实时成像的吞吐量。因此，传统SPI不适用于高速、高分辨率任务。

为了解决这些挑战，研究组开发了一种利用频率梳光相干编码（FACE）的超高通量单像素复杂场显微镜（SPCM）系统。该系统可实现非可见域的实时复杂场监控。在1030 nm波长下，实现了创纪录的空间-带宽-时间积 (SBP-T) 1.3 × 10⁷，超越了先前的单点计数模块 (SPCM，约 10⁴)、单像素成像 (SPI，约 10⁵)，甚至某些类型的商用近红外相机 (约 10⁶)。

该系统支持以1000Hz的帧率进行实时流传输，帧尺寸为 80 × 81 像素，在大约 300 微米的视场范围内提供 3.76 微米的横向分辨率。研究组通过对动态透明场景（包括微流体、活体微生物、化学反应）以及穿透散射介质成像，验证了该系统的性能。这一进展为非可见光波段的高速、高分辨率复振幅场成像提供了一种更优的解决方案，显著提升了单像素成像 (SPI) 在各种应用中的性能。

附：英文原文

Title: Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)

Author: Wu, Daixuan, Shen, Yuecheng, Zhu, Zhongzheng, Li, Tijian, Luo, Jiawei, Wang, Zhengyang, Liang, Jiaming, Zhang, Zhiling, Yao, Yunhua, Qi, Dalong, Deng, Lianzhong, Sun, Zhenrong, Liu, Meng, Luo, Zhi-Chao, Zhang, Shian

Issue&Volume: 2025-08-11

Abstract: Single-pixel imaging (SPI) is a promising technology for optical imaging beyond the visible spectrum, where commercial cameras are expensive or unavailable. However, limitations such as slow pattern projection rates and time-consuming reconstruction algorithms hinder its throughput for real-time imaging. Consequently, conventional SPI is inadequate for high-speed, high-resolution tasks. To address these challenges, we developed an ultrahigh-throughput single-pixel complex-field microscopy (SPCM) system utilizing frequency-comb acousto-optic coherent encoding (FACE). This system enables real-time complex-field monitoring in the non-visible domain. Operating at 1030nm, our system achieves a record-high space-bandwidth-time product (SBP-T) of 1.3×107, surpassing previous SPCM (~104), SPI (~105), and even certain types of commercial near-infrared cameras (~106). It supports real-time streaming at 1000Hz with a frame size of 80×81 pixels and a lateral resolution of 3.76μm across an approximately 300μm field of view. We validated the system by imaging dynamic transparent scenes, including microfluidics, live microorganisms, chemical reactions, as well as imaging through scattering media. This advancement offers a superior solution for high-speed, high-resolution complex-field imaging beyond the visible spectrum, significantly enhancing SPI performance across various applications.

DOI: 10.1038/s41377-025-01931-w

Source: https://www.nature.com/articles/s41377-025-01931-w