清华大学苑克鑫小组的研究开发出了VIVIT：通过离子玻璃组织解析跨尺度体积生物结构。相关论文发表在2025年8月11日出版的《细胞》杂志上。
小组介绍了基于玻璃离子-液体-溶剂的跨尺度生物结构体积检测（VIVIT），这是一种利用离子液体化学性质的三维组织学方法。VIVIT将生物组织转化为离子玻璃态，从而实现最小畸变和高透明度的光学清除，保护组织免受低温晶体损伤，并放大来自遗传编码和免疫染色标记的荧光信号，从而在完整的3D结构中产生精确可靠的荧光信号映射。使用VIVIT，研究组证明了突触输入到多感觉丘脑神经元的方式与它们的全脑输出目标之间的联系，并确定了人类皮层中抑制控制的各个方面。VIVIT为阐明跨尺度生物结构的组织原理提供了机会。
据介绍，跨尺度的生物结构无缝集成以执行基本功能。虽然已经开发了各种组织学方法来揭示这些复杂的结构，但在高分辨率揭示微观结构的同时保持大体积结构的完整性仍然是一个主要挑战。
附：英文原文
Title: VIVIT: Resolving trans-scale volumetric biological architectures via ionic glassy tissue
Author: Yixiao Gao, Fengyuan Xin, Tao Wang, Chengjun Shao, Ying Hu, Zhuoya Chen, Yiwei Wang, Fenghua Xie, Tianyu Li, Sijie Li, Liqun Ren, Caiqin Li, Xian Yang, Zhongjun Yang, Meijie Li, KaMun Tan, Tao Bai, Changwei Wei, Hanchuan Peng, Kun Li, Yichang Jia, Kexin Yuan
Issue&Volume: 2025-08-11
Abstract: Biological structures across scales integrate seamlessly to perform essential functions. While various histological methods have been developed to reveal these intricate structures, preserving the integrity of large-volume architectures while revealing microstructures with high resolution remains a major challenge. Here, we introduce vitreous ionic-liquid-solvent-based volumetric inspection of trans-scale biostructure (VIVIT), a 3D histological method leveraging the chemical properties of ionic liquids. VIVIT transforms biological tissue into an ionic glassy state, which enables optical clearing with minimal distortion and high transparency, preserves tissue from low-temperature crystal damage, and amplifies fluorescent signals from both genetically encoded and immunostained labels, thus yielding precise and reliable mapping of fluorescent signals within intact 3D architectures. Using VIVIT, we demonstrate the link between the modality of synaptic inputs to multisensory thalamic neurons and the targets of their brain-wide outputs and identified aspects of inhibitory control in the human cortex. VIVIT thus offers opportunities to elucidate the organizational principles underlying trans-scale biostructures.
DOI: 10.1016/j.cell.2025.07.023
Source: https://www.cell.com/cell/abstract/S0092-8674(25)00813-X