北京大学汪阳明团队宣布他们的最新研究提出了单细胞和组织中原位RNA-蛋白相互作用和转录组的共谱分析。相关论文于2025年8月11日发表在《自然—方法学》杂志上。

在这里，该团队引入了MAPIT-seq（添加到RBP相互作用转录测序中的修饰），这是一种抗体导向的编辑策略，可以同时绘制全基因组的RBP-RNA相互作用和基因表达。该课题组研究人员证明了MAPIT-seq在多个RBP中的稳定性，并系统地分析了与核心多梳抑制复合体2（PRC2）成分相关的RNA底物。MAPIT-seq也适用于冷冻组织切片，可以绘制RBP在大脑发育过程中的作用。重要的是，该课题组人员开发了高通量单细胞MAPIT-seq （scMAPIT-seq）来揭示细胞阶段特异性RBP调控。总之，MAPIT-seq扩展了多组学分析，为研究动态生物学过程和临床相关场景中的转录后调控提供了一个有效的框架。

据了解，RNA结合蛋白（RBP）是RNA命运和功能的重要调节因子。研究RBP调控的一个长期挑战是在动态转录组学景观中绘制RNA相互作用组，特别是在单细胞环境和初级组织中。

附：英文原文

Title: Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues

Author: Cheng, Qi-Xuan, Xie, Gang, Zhang, Xiangyu, Wang, Jie, Ding, Shuangjin, Wu, Yi-Xia, Shi, Ming, Duan, Fei-Fei, Wan, Zi-Li, Wei, Jing-Jia, Xiao, Junyu, Wang, Yangming

Issue&Volume: 2025-08-11

Abstract: RNA-binding proteins (RBPs) are essential regulators of RNA fate and function. A long-standing challenge in studying RBP regulation has been mapping RNA interactomes within the dynamic transcriptomic landscape, especially in single-cell contexts and primary tissues. Here we introduce MAPIT-seq (modification added to RBP interacting transcript-sequencing), which uses an antibody-directed editing strategy to map genome-wide in situ RBP–RNA interactions and gene expression concurrently. We demonstrate MAPIT-seq’s robustness across multiple RBPs and systematically analyze RNA substrates associated with core polycomb repressive complex 2 (PRC2) components. MAPIT-seq is also applicable to frozen tissue sections, enabling the mapping of RBP roles during brain development. Importantly, we develop high-throughput single-cell MAPIT-seq (scMAPIT-seq) to reveal cell stage-specific RBP regulation. In summary, MAPIT-seq expands multi-omics profiling, providing an effective framework to study post-transcriptional regulation in dynamic biological processes and clinically relevant scenarios.

DOI: 10.1038/s41592-025-02774-4

Source: https://www.nature.com/articles/s41592-025-02774-4