福州大学杨黄浩团队的研究开发出了快速、成本效益和灵活的DNA测序卷对卷流体。2025年7月14日出版的《自然—方法学》杂志发表了这项成果。

然而，近年来，由于流细胞中的泊泽维尔流体动力学的基本限制，性能改进已经放缓，研究小组克服了这些限制，主题是Couette流。

在这里，课题组通过卷对卷流体（r2r-fl）展示了NGS，这是一种与柔性生物芯片尺寸兼容的具有成本效益的方法。r2r-fl是平面Couette流程的实际实现，试剂消耗量降低85倍（每千兆酶对0.16美元），冲洗次数低于2S和对端100碱基对测序周转时间从几天减少到少于12h。该方法对人类基因组单核苷酸多态性的检测精度保持在99.9%以上，灵敏度保持在99.3%以上，对大肠杆菌的定位率保持在99.9%以上，对严重急性呼吸综合征冠状病毒2 α株的核苷酸替换、缺失或插入保持在最低水平。通过降低成本和时间，r2r-fl可实现快速、可扩展的NGS，用于病原体检测、癌症诊断和遗传疾病分析。

据悉，新一代测序（NGS）技术在生物学研究和临床应用方面都取得了显著成功。

附：英文原文

Title: Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics

Author: Qin, Yanzhe, Koehler, Stephan A., Ling, Yunyan, Yu, Shiqiang, Zhang, Yongjie, Luo, Jie, Chen, Kaijian, Junjie, Luo, Zeng, Junjie, Chu, Haiyang, Wang, Fei, Li, Wei, Li, Dan, Yu, Xinran, Wu, Xiangchao, Zhao, Shengming, Lu, Hao, Deng, Ziqing, Yang, Zhijian, Mai, Ruibin, Liu, Zhuo, Niu, Zihua, Huang, Xin, Xing, Chengmei, Zhang, Xingcai, Hu, Yongyou, Peng, Xinwen, Liao, Xiaoxing, Qu, Xiangmeng, Gong, Yiming, Chen, Qiushui, Hermans, Thomas M., Zhang, Wenwei, Yang, Huanghao

Issue&Volume: 2025-07-14

Abstract: Next-generation sequencing (NGS) technologies have achieved remarkable success in both biological research and clinical applications. However, in recent years, performance improvements have slowed due to fundamental limitations imposed by Poiseuille fluid dynamics in flow cells, which we overcome using Couette flow. Here we show NGS by roll-to-roll fluidics (r2r-fl), a cost-effective approach compatible with flexible biochip sizes. r2r-fl is a practical implementation of plane Couette flow, with up to 85-fold lower reagent consumption (US$0.16 per gigabase pair), rinsing times under 2s and a reduction in paired-end 100-base pair sequencing turnaround from days to less than 12h. The method maintains over 99.9% precision and 99.3% sensitivity of single nucleotide polymorphisms in the human genome, 99.9% mapping rate for Escherichia coli, and minimal nucleotide substitutions, deletions or insertions in the severe acute respiratory syndrome coronavirus 2 alpha strain. By lowering cost and time, r2r-fl enables rapid, scalable NGS for pathogen detection, cancer diagnostics and genetic disease profiling.

DOI: 10.1038/s41592-025-02730-2

Source: https://www.nature.com/articles/s41592-025-02730-2