中国科学院脑科学与智能技术卓越创新中心毕国强研究组报道了亚细胞分辨率下全鼠周围神经的高速制图。2025年7月10日，国际知名学术期刊《细胞》发表了这一成果。
研究组提出了一种具有优化的全身清除工作流程的高速块面体积成像系统，能够在40小时内以微米分辨率成像整个成年motheme。Thy1-EGFP小鼠单个脊髓纤维的三维重建显示了沿腹侧和背侧分支的感觉和运动投射的明显形态学特征。免疫染色有助于交感神经及其分支在全身范围内的定位，突出其在肢体肌肉、骨骼和大多数内脏器官的血管周围模式。病毒追踪阐明了迷走神经和单个迷走神经纤维的精细结构，揭示了各种器官意想不到的投射路线。他们的方法提供了一种有效的方法，以实现对不同系统之间的细胞水平相互作用的整体理解，这些系统是身体生理和疾病的基础。
研究人员表示，与哺乳动物大脑中尺度连接体图谱的快速发展相比，由于其体型和复杂性，周围神经系统的类似图谱仍然具有挑战性。
附：英文原文
Title: High-speed mapping of whole-mouse peripheral nerves at subcellular resolution
Author: Mei-Yu Shi, Yuchen Yao, Miao Wang, Qi Yang, Lufeng Ding, Rui Li, Yuanyuan Li, Haimeng Huang, Chao-Yu Yang, Zhao Zhou, Zhenxiang Zhu, Pengjie Wen, Fangling Dai, Xiaohui Zeng, Ke-Ming Zhang, Yuhong Guo, Zi-An Sun, Huanhuan Xia, Zhenhua Ren, Yusuf Ozgur Cakmak, Ming Zhang, Fuqiang Xu, Lei Qu, Qingyuan Zhu, Pak-Ming Lau, Cheng Xu, Guo-Qiang Bi
Issue&Volume: 2025/07/10
Abstract: In contrast to the rapid advancements in mesoscale connectomic mapping of the mammalian brain, similar mapping of the peripheral nervous system has remained challenging due to the body size and complexity. Here, we present a high-speed blockface volumetric imaging system with an optimized workflow of whole-body clearing, capable of imaging the entire adult mouse at micrometer resolution within 40 h. Three-dimensional reconstruction of individual spinal fibers in Thy1-EGFP mice reveals distinct morphological features of sensory and motor projections along the ventral and dorsal rami. Immunostaining facilitates body-wide mapping of sympathetic nerves and their branches, highlighting their perivascular patterns in limb muscles, bones, and most visceral organs. Viral tracing elucidates the fine architecture of vagus nerves and individual vagal fibers, revealing unexpected projection routes to various organs. Our approach offers an effective means to achieve a holistic understanding of cellular-level interactions among different systems that underlie body physiology and disease.
DOI: 10.1016/j.cell.2025.06.011
Source: https://www.cell.com/cell/abstract/S0092-8674(25)00673-7