利用2P成像和全细胞电生理技术，课题组研究人员确定了DA传递到纹状体间接通路棘突神经元的时空特性。DA释放位点的稀疏激活引起局部DA信号，产生空间离散的D2受体介导的树突反应。DA受体信号的时空特性在下游细胞内通路之间存在差异。该团队提出膜界Gβγ信号与细胞内第二信使通路平行发生，但在不同的空间和时间尺度上，为纹状体神经元精确解码DA信号提供了一种机制。
据了解，多巴胺（DA）传递通过激活不同亚型的G蛋白偶联受体，对多种基底神经节依赖性行为进行关键调控。空间上广泛的DA释放已被广泛表征，但DA传递如何在亚细胞尺度上影响纹状体功能仍然知之甚少。
附：英文原文
Title: Discrete spatiotemporal encoding of striatal dopamine transmission
Author: Andrew G. Yee, Yini Liao, Brian S. Muntean, Christopher P. Ford
Issue&Volume: 2025-07-10
Abstract: Dopamine (DA) transmission critically regulates diverse, basal ganglia–dependent behaviors by activating distinct subtypes of G protein–coupled receptors. Spatially broad DA release has been extensively characterized, but how DA transmission affects striatal function at a subcellular scale remains poorly understood. Using 2P imaging and whole-cell electrophysiology, we defined the spatiotemporal properties of DA transmission onto striatal indirect pathway spiny projection neurons. Sparse activation of DA release sites evoked localized DA signals, producing spatially discrete, D2 receptor–mediated responses across dendrites. The spatiotemporal properties of DA receptor signaling differed between downstream intracellular pathways. We propose that membrane-delimited Gβγ signaling occurs in parallel to intracellular second messenger pathways, but on different spatial and temporal scales, providing a mechanism for precise decoding of DA signals by striatal neurons.
DOI: adp9833
Source: https://www.science.org/doi/10.1126/science.adp9833