加拿大萨斯喀彻温大学Curtis J. Pozniak小组近日取得一项新成果。经过不懈努力，他们的研究开发出了配对NLRs的协同作用决定了Yr84介导的小麦抗条锈病能力。相关论文于2025年6月9日发表在《自然—遗传学》杂志上。

本文报道了野生二粒小麦（Triticum turgidum ssp）的两个基因的克隆。基于精细定位、长读测序和突变诱导功能验证的Yr84介导的条纹线虫抗性基础。与之前克隆的所有条锈病基因不同，不完全显性的Yr84表型是通过配对的核苷酸结合富亮氨酸重复序列（NLR）基因CNL和NL的协调功能赋予的。该团队认为，基于它们的基因组组织、注释、表达谱和预测的蛋白质结构，CNL作为传感器NLR，负责效应物识别，而NL作为辅助NLR，启动下游的抗性级联。CNL和NL都缺乏先前与成对NLR的效应识别相关的集成域；因此，这些发现有助于深入了解植物配对NLRs的结构和功能的分子机制。

研究人员表示，抗性基因的克隆扩展了对抗性基因功能的认识，并促进了抗性基因在育种中的应用。

附：英文原文

Title: Coordinated function of paired NLRs confers Yr84-mediated stripe rust resistance in wheat

Author: Klymiuk, Valentyna, Wiebe, Krystalee, Chawla, Harmeet Singh, Ens, Jennifer, Subramaniam, Rajagopal, Pozniak, Curtis J.

Issue&Volume: 2025-06-09

Abstract: Cloning of resistance genes expands our understanding of their function and facilitates their deployment in breeding. Here we report the cloning of two genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) underlying Yr84-mediated stripe rust resistance using a combination of fine mapping, long-read sequencing and mutation-induced functional validation. In contrast to all previously cloned stripe rust genes, the incompletely dominant Yr84 phenotype is conferred through the coordinated function of paired nucleotide-binding leucine-rich repeat (NLR) genes CNL and NL. We reason that based on their genomic organization, annotation, expression profiles and predicted protein structure, CNL functions as a sensor NLR, responsible for effector recognition, and NL acts as a helper NLR, initiating downstream resistance cascades. Both the CNL and NL lack an integrated domain(s) previously implicated in effector recognition by paired NLRs; therefore, these findings contribute insights into the structure and molecular mechanisms of the function of plant paired NLRs.

DOI: 10.1038/s41588-025-02203-4

Source: https://www.nature.com/articles/s41588-025-02203-4