小组通过传递合成肽-morpholino嵌合体，沉默了活体细胞中特定线粒体mRNA的翻译。这种方法使他们能够对细胞反应进行全面的时间监测。他们的研究为线粒体翻译及其与细胞生理学的整合提供了见解，并提供了一种解决活细胞中线粒体基因表达的策略。该方法可以潜在地以分析线粒体基因表达在一系列细胞模型系统中的机制和病理生理学为主题。
据了解，线粒体在代谢和能量供应中起着中心作用。它们表达自己的基因组，编码氧化磷酸化系统的关键亚基。然而，线粒体基因表达的核心机制仍然是个谜。缺乏合适的技术来靶向线粒体蛋白在细胞中的合成，限制了实验的进入。
附：英文原文
Title: Silencing mitochondrial gene expression in living cells
Author: Luis Daniel Cruz-Zaragoza, Drishan Dahal, Mats Koschel, Angela Boshnakovska, Aiturgan Zheenbekova, Mehmet Yilmaz, Marcel Morgenstern, Jan-Niklas Dohrke, Julian Bender, Anusha Valpadashi, Kristine A Henningfeld, Silke Oeljeklaus, Laura Sophie Kremer, Mirjam Breuer, Oliver Urbach, Sven Dennerlein, Michael Lidschreiber, Stefan Jakobs, Bettina Warscheid, Peter Rehling
Issue&Volume: 2025-05-22
Abstract: Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms underlying mitochondrial gene expression remain enigmatic. A lack of suitable technologies to target mitochondrial protein synthesis in cells has limited experimental access. Here, we silenced the translation of specific mitochondrial mRNAs in living human cells by delivering synthetic peptide-morpholino chimeras. This approach allowed us to perform a comprehensive temporal monitoring of cellular responses. Our study provides insights into mitochondrial translation, its integration into cellular physiology, and provides a strategy to address mitochondrial gene expression in living cells. The approach can potentially be used to analyze mechanisms and pathophysiology of mitochondrial gene expression in a range of cellular model systems.
DOI: adr3498
Source: https://www.science.org/doi/10.1126/science.adr3498