人类生物学研究所J. Gray Camp课题组近日取得一项新成果。经过不懈努力，他们开发出人类内胚层来源类器官的整合转录组细胞图谱。该研究于2025年5月12日发表于国际一流学术期刊《自然—遗传学》杂志上。

研究人员整合了来自218个样本的单细胞转录组，这些样本涵盖了类器官和其他多种内胚层来源的组织模型，以建立一个初步版本的人类内胚层来源的类器官细胞图谱。该集成包括跨越不同条件、数据强度和协议的近100万个单元。课题组研究人员比较了类器官模型之间的细胞类型和状态，并通过映射到初级组织对应物来协调细胞注释。聚焦于肠和肺，该团队提供了来自新方案的制图数据示例，并展示了如何将图谱作为一个多样化的队列来评估扰动和疾病模型。人类内胚层来源的类器官细胞图谱使各种数据集集中可用，对评估保真度、表征紊乱和病变状态以及简化方案制定具有价值。

研究人员表示，人类多能干细胞和组织驻留胚胎和成体干细胞可以在体外产生内胚层起源的上皮组织，再现发育和成人生理学的各个方面。

附：英文原文

Title: An integrated transcriptomic cell atlas of human endoderm-derived organoids

Author: Xu, Quan, Halle, Lennard, Hediyeh-zadeh, Soroor, Kuijs, Merel, Riedweg, Rya, Kilik, Umut, Recaldin, Timothy, Yu, Qianhui, Rall, Isabell, Frum, Tristan, Adam, Lukas, Parikh, Shrey, Kfuri-Rubens, Raphael, Gander, Manuel, Klein, Dominik, Curion, Fabiola, He, Zhisong, Fleck, Jonas Simon, Oost, Koen, Kahnwald, Maurice, Barbiero, Silvia, Mitrofanova, Olga, Maciag, Grzegorz Jerzy, Jensen, Kim B., Lutolf, Matthias, Liberali, Prisca, Spence, Jason R., Gjorevski, Nikolche, Beumer, Joep, Treutlein, Barbara, Theis, Fabian J., Camp, J. Gray

Issue&Volume: 2025-05-12

Abstract: Human pluripotent stem cells and tissue-resident fetal and adult stem cells can generate epithelial tissues of endodermal origin in vitro that recapitulate aspects of developing and adult human physiology. Here, we integrate single-cell transcriptomes from 218 samples covering organoids and other models of diverse endoderm-derived tissues to establish an initial version of a human endoderm-derived organoid cell atlas. The integration includes nearly one million cells across diverse conditions, data sources and protocols. We compare cell types and states between organoid models and harmonize cell annotations through mapping to primary tissue counterparts. Focusing on the intestine and lung, we provide examples of mapping data from new protocols and show how the atlas can be used as a diverse cohort to assess perturbations and disease models. The human endoderm-derived organoid cell atlas makes diverse datasets centrally available and will be valuable to assess fidelity, characterize perturbed and diseased states, and streamline protocol development.

DOI: 10.1038/s41588-025-02182-6

Source: https://www.nature.com/articles/s41588-025-02182-6