隆德大学Johan Malmström课题组取得一项新突破。他们研究出用于组织特异性血浆蛋白质组动力学的人类蛋白质组分布图谱。这一研究成果于2025年4月8日发表在国际顶尖学术期刊《细胞》上。

为了量化不同器官和细胞在健康和患病条件下对血浆蛋白质组的影响程度，研究人员开发了一种基于质谱的蛋白质组学策略来推断在人类血浆中检测到的蛋白质的组织来源。该研究团队首先从18个血管化器官和血液中8种最丰富的细胞类型构建了一个广泛的人类蛋白质组图谱。该图谱与先前的RNA和蛋白质图谱相结合，客观地定义蛋白质组范围内的蛋白质与器官的关联，以推断起源，并使血浆中器官特异性蛋白的可重复性定量。课题组证明抵抗可以确定六个独立患者队列中器官富集蛋白面板的疾病特异性定量变化，包括败血症，胰腺炎和心肌损伤。该策略可以扩展到其他疾病，以促进他们对有助于血浆蛋白质组动力学的过程的理解。

据了解，血浆蛋白质组是由周围器官和细胞的蛋白质流入和流出来维持的。

附：英文原文

Title: Human proteome distribution atlas for tissue-specific plasma proteome dynamics

Author: Erik Malmstrm, Lars Malmstrm, Simon Hauri, Tirthankar Mohanty, Aaron Scott, Christofer Karlsson, Carlos Gueto-Tettay, Emma hrman, Shahab Nozohoor, Bobby Tingstedt, Sara Regner, Peter Elfving, Leif Bjermer, Andreas Forsvall, Alexander Doyle, Mattias Magnusson, Ingrid Hedenfalk, Pivi Kannisto, Christian Brandt, Emma Nilsson, Lars B. Dahlin, Johan Malm, Adam Linder, Emma Niméus, Johan Malmstrm

Issue&Volume: 2025-04-08

Abstract: The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs and cells. To quantify the extent to which different organs and cells impact the plasma proteome in healthy and diseased conditions, we developed a mass-spectrometry-based proteomics strategy to infer the tissue origin of proteins detected in human plasma. We first constructed an extensive human proteome atlas from 18 vascularized organs and the 8 most abundant cell types in blood. The atlas was interfaced with previous RNA and protein atlases to objectively define proteome-wide protein-organ associations to infer the origin and enable the reproducible quantification of organ-specific proteins in plasma. We demonstrate that the resource can determine disease-specific quantitative changes of organ-enriched protein panels in six separate patient cohorts, including sepsis, pancreatitis, and myocardial injury. The strategy can be extended to other diseases to advance our understanding of the processes contributing to plasma proteome dynamics.

DOI: 10.1016/j.cell.2025.03.013

Source: https://www.cell.com/cell/abstract/S0092-8674(25)00286-7