近日，中国科学院生物物理研究所李栋及其课题组研究出用于快速、长期、近各向同性亚细胞成像的快速自适应超分辨率晶格光片显微镜。2025年4月29日出版的《自然—方法学》发表了这项成果。

在这里，研究团队提出了基于元学习的反射晶格光片虚拟结构照明显微镜（Meta-rLLS-VSIM），它将晶格光片显微镜升级到接近各向同性的超分辨率~120横向Nm和~160Nm轴向，无需修改核心光学系统或损失其他活细胞成像指标。

此外，该课题组人员设计了一种前端成像系统和后端元学习框架协同的自适应在线训练方法，将对训练数据的需求减少了10倍，将数据采集和模型训练的总时间缩短到数十秒。研究人员展示了Meta-rLLS-VSIM的多功能，通过对数百个多色体积的各种生物过程进行超高时空分辨率成像，描绘了胚胎和真核细胞中多个细胞器的纳米级分布、动力学和相互作用模式。

据了解，晶格光片显微镜提供了一个重要的观察窗口，进入细胞内和细胞间生理的活标本，但在衍射有限的分辨率或各向异性超分辨率与结构照明。

附：英文原文

Title: Fast-adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging

Author: Qiao, Chang, Li, Ziwei, Wang, Zongfa, Lin, Yuhuan, Liu, Chong, Zhang, Siwei, Liu, Yong, Feng, Yun, Yang, Xiaoyu, Fu, Wenfeng, Dong, Xue, Guo, Jiabao, Xu, Wencong, Wang, Xinyu, Jiang, Tao, Meng, Quan, Wang, Qinghua, Dai, Qionghai, Li, Dong

Issue&Volume: 2025-04-29

Abstract: Lattice light-sheet microscopy provides a crucial observation window into intra- and intercellular physiology of living specimens but at the diffraction-limited resolution or anisotropic super-resolution with structured illumination. Here we present meta-learning-empowered reflective lattice light-sheet virtual structured illumination microscopy (Meta-rLLS-VSIM), which upgrades lattice light-sheet microscopy to a near-isotropic super resolution of ~120nm laterally and ~160nm axially without modifications of the core optical system or loss of other live-cell imaging metrics. Moreover, we devised an adaptive online training approach by synergizing the front-end imaging system and back-end meta-learning framework, which alleviated the demand for training data by tenfold and reduced the total time for data acquisition and model training down to tens of seconds. We demonstrate the versatile functionalities of Meta-rLLS-VSIM by imaging a variety of bioprocesses with ultrahigh spatiotemporal resolution for hundreds of multicolor volumes, delineating the nanoscale distributions, dynamics and interaction patterns of multiple organelles in embryos and eukaryotic cells.

DOI: 10.1038/s41592-025-02678-3

Source: https://www.nature.com/articles/s41592-025-02678-3