一种与基质辅助激光解吸/电离质谱成像（TEMI）兼容的组织扩增方法，这一成果由霍华德休斯医学院研究小组经过不懈努力而取得。这一研究成果发表在2025年4月22日出版的国际学术期刊《自然—方法学》上。

在这里，课题组开发了一种与基质辅助激光解吸/电离质谱成像（TEMI）兼容的组织扩增方法。TEMI在不牺牲体素吞吐量的情况下达到单细胞空间分辨率，并能够分析数百种生物分子，包括脂质，代谢物，肽（蛋白质）和N-聚糖。利用TEMI，研究团队绘制了生物分子在各种哺乳动物组织中的空间分布，并揭示了肿瘤中的代谢异质性。TEMI可以很容易地适应并广泛应用于生物和医学研究，以推进空间多组学分析。

研究人员表示，多细胞生物体内多种生物分子的空间分布对其生理功能至关重要。生物分子的高通量原位作图对于基础研究和医学研究都至关重要，需要高扫描速度、空间分辨率和化学灵敏度。

附：英文原文

Title: TEMI: tissue-expansion mass-spectrometry imaging

Author: Zhang, Hua, Ding, Lang, Hu, Amy, Shi, Xudong, Huang, Penghsuan, Lu, Haiyan, Tillberg, Paul W., Wang, Meng C., Li, Lingjun

Issue&Volume: 2025-04-22

Abstract: The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling.

DOI: 10.1038/s41592-025-02664-9

Source: https://www.nature.com/articles/s41592-025-02664-9