法国里昂大学Teva Vernoux研究组开发出生长素依赖性转录码的合成反褶积。2025年4月15日出版的《细胞》杂志发表了这项成果。
该研究团队为植物的主要调节因子生长素解决了这个问题。生长素的转录应答依赖于一个大的多基因转录因子家族，即生长素应答因子（ARFs）。研究团队解开了ARF调控的转录主题生长素诱导的合成启动子的复杂性，这些启动子是由ARF差异结合的顺式元件对结构构建的。课题组研究人员向细胞系统证明，ARF转录特性不仅是内在的，而且还取决于它们所结合的顺式元件对结构，它们识别出一个双层ARF/顺式元件转录代码。生长素诱导的合成启动子在植物中表达差异，在单细胞分辨率下显示了这种双层编码如何模式对生长素的转录反应。在合成启动子中结合顺式元件对结构创建了不同的模式，证明了生长素双层编码在产生多种基因表达模式方面的组合能力，而这些基因表达模式不仅仅是生长素分布的直接翻译。
据悉，发育信号如何在空间和时间上调控基因表达仍然知之甚少。
附：英文原文
Title: Synthetic deconvolution of an auxin-dependent transcriptional code
Author: Raquel Martin-Arevalillo, Bruno Guillotin, Jonas Schn, Alice Hugues, Marie-France Gerentes, Kun Tang, Jérémy Lucas, Emmanuel Thévenon, Marianne Dreuillet, Graeme Vissers, Mohammed Mohammed Ateequr, Carlos S. Galvan-Ampudia, Guillaume Cerutti, Jonathan Legrand, Coralie Cance, Annick Dubois, Franois Parcy, Kenneth D. Birnbaum, Matias D. Zurbriggen, Renaud Dumas, Franois Roudier, Teva Vernoux
Issue&Volume: 2025-04-15
Abstract: How developmental signals program gene expression in space and time is still poorly understood. Here, we addressed this question for the plant master regulator, auxin. Transcriptional responses to auxin rely on a large multigenic transcription factor family, the auxin response factors (ARFs). We deconvoluted the complexity of ARF-regulated transcription using auxin-inducible synthetic promoters built from cis-element pair configurations differentially bound by ARFs. We demonstrate using cellular systems that ARF transcriptional properties are not only intrinsic but also depend on the cis-element pair configurations they bind to, thus identifying a bi-layer ARF/cis-element transcriptional code. Auxin-inducible synthetic promoters were expressed differentially in planta showing at single-cell resolution how this bi-layer code patterns transcriptional responses to auxin. Combining cis-element pair configurations in synthetic promoters created distinct patterns, demonstrating the combinatorial power of the auxin bi-layer code in generating diverse gene expression patterns that are not simply a direct translation of auxin distribution.
DOI: 10.1016/j.cell.2025.03.028
Source: https://www.cell.com/cell/abstract/S0092-8674(25)00346-0