2025年4月11日出版的《科学》杂志发表了中国科学院微生物研究所
在这项工作中，该研究组报道了柑橘中一个由E3泛素连接酶PUB21及其底物MYC2转录因子组成的HLB抗性调控回路，该回路调节茉莉酸介导的防御反应。在PUB21启动子中的helitron插入引入了多个MYC2结合的顺式元件，从而创建了一个将PUB21活性与MYC2降解联系起来的调控回路。在柑橘远亲中发现的自然显性阴性PUB21同源异位表达稳定了MYC2并赋予了对HLB的抗性。人工智能鉴定的抗蛋白水解肽（APPs）通过结合和抑制PUB21活性来稳定MYC2。一种由14个氨基酸组成的肽APP3-14在温室和田间试验中被分子控制。这种方法代表了一种通过靶向抗病蛋白稳定来对抗不可培养病原体的策略。
研究人员表示，黄龙病（HLB）是一种毁灭性的柑橘病。
附：英文原文
Title: Targeted MYC2 stabilization confers citrus Huanglongbing resistance
Author: Pingzhi Zhao, Huan Yang, Yanwei Sun, Jingyin Zhang, Kaixing Gao, Jinbao Wu, Chengrong Zhu, Cece Yin, Xiaoyue Chen, Qi Liu, Qiudong Xia, Qiong Li, Han Xiao, Hai-Xi Sun, Xiaoxiao Zhang, Long Yi, Changyong Zhou, Daniel J. Kliebenstein, Rongxiang Fang, Xuefeng Wang, Jian Ye
Issue&Volume: 2025-04-11
Abstract: Huanglongbing (HLB) is a devastating citrus disease. In this work, we report an HLB resistance regulatory circuit in Citrus composed of an E3 ubiquitin ligase, PUB21, and its substrate, the MYC2 transcription factor, which regulates jasmonate-mediated defense responses. A helitron insertion in the PUB21 promoter introduced multiple MYC2-binding cis-elements to create a regulatory circuit linking the PUB21 activity with MYC2 degradation. Ectopic expression of a natural dominant-negative PUB21 paralog discovered in distant Citrus relatives stabilized MYC2 and conferred resistance to HLB. Antiproteolysis peptides (APPs), identified by artificial intelligence, stabilized MYC2 by binding and inhibiting PUB21 activity. A 14–amino acid peptide, APP3-14, molecularly controlled HLB in greenhouse and field trials. This approach represents a strategy to combat uncultivable pathogens through targeted disease resistance protein stabilization.
DOI: adq7203
Source: https://www.science.org/doi/10.1126/science.adq7203