串联激酶蛋白是谷类植物先天免疫系统的基础，但它们如何启动植物免疫反应尚不清楚。该报告鉴定了小麦蛋白串联NBD 1 (WTN1)，这是一种非典型核苷酸结合富亮氨酸重复（NLR）受体，具有串联核苷酸结合适配器，与APAF-1、植物R蛋白和CED-4 （NB-ARC）结构域共享，是WTK3介导的抗病性所必需的。WTK3及其等位基因变体Rwt4（已知分别具有小麦白粉病和稻瘟病的抗性）都能够识别稻瘟病效应物PWT4。它们激活WTN1形成钙渗透通道，类似于ZAR1和Sr35。它们串联激酶蛋白和它们相关的NLRs作为“传感器-执行器”对对抗真菌病原体。此外，进化分析揭示了串联激酶-NLR模块的共同进化轨迹，突出了它们在触发植物免疫中的协同作用。
附：英文原文
Title: A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens
Author: Ping Lu, Gaohua Zhang, Jing Li, Zhen Gong, Gaojie Wang, Lingli Dong, Huaizhi Zhang, Guanghao Guo, Min Su, Ke Wang, Yueming Wang, Keyu Zhu, Qiuhong Wu, Yongxing Chen, Miaomiao Li, Baoge Huang, Beibei Li, Wenling Li, Lei Dong, Yikun Hou, Xuejia Cui, Hongkui Fu, Dan Qiu, Chengguo Yuan, Hongjie Li, Jian-Min Zhou, Guan-Zhu Han, Yuhang Chen, Zhiyong Liu
Issue&Volume: 2025-03-28
Abstract: Tandem kinase proteins underlie the innate immune systems of cereal plants, but how they initiate plant immune responses remains unclear. This report identifies wheat protein wheat tandem NBD 1 (WTN1), a noncanonical nucleotide-binding leucine-rich repeat (NLR) receptor featuring tandem nucleotide binding adaptor shared by APAF-1, plant R proteins, and CED-4 (NB-ARC) domains, required for WTK3-mediated disease resistance. Both WTK3 and its allelic variant Rwt4—known for conferring resistance to wheat powdery mildew and blast, respectively—are capable of recognizing the blast effector PWT4. They activate WTN1 to form calcium-permeable channels, akin to ZAR1 and Sr35. Thus, tandem kinase proteins and their associated NLRs operate as “sensor-executor” pairs against fungal pathogens. Additionally, evolutionary analyses reveal a coevolutionary trajectory of the tandem kinase-NLR module, highlighting their cooperative role in triggering plant immunity.
DOI: adp5469
Source: https://www.science.org/doi/10.1126/science.adp5469