线粒体呼吸链的细胞内结构，这一成果由
研究小组利用原位冷冻电子断层成像技术来观察莱茵衣藻细胞中几种主要线粒体复合物的天然结构和组织。ATP合酶和呼吸复合物分别分离到弯曲和平坦的嵴膜结构域。呼吸复合体I、III和IV组装成一个呼吸小体超复合体，从中研究小组确定了一个天然的5埃分辨率结构显示电子载体细胞色素C的结合。结合单粒子低温电镜在2.4-决议，研究人员模拟呼吸复合体如何组织在天然线粒体。
据了解，线粒体通过氧化磷酸化再生三磷酸腺苷（ATP）。这个过程是由五种膜结合复合物共同完成的，这些复合物被称为呼吸链，它们协同工作来转移电子和泵送质子。这些复合物在原生细胞中的精确组织是有争议的。
附：英文原文
Title: In-cell architecture of the mitochondrial respiratory chain
Author: Florent Waltz, Ricardo D. Righetto, Lorenz Lamm, Thalia Salinas-Giegé, Ron Kelley, Xianjun Zhang, Martin Obr, Sagar Khavnekar, Abhay Kotecha, Benjamin D. Engel
Issue&Volume: 2025-03-21
Abstract: Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo–electron tomography to visualize the native structures and organization of several major mitochondrial complexes in Chlamydomonas reinhardtii cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom () resolution structure showing binding of electron carrier cytochrome c. Combined with single-particle cryo–electron microscopy at 2.4- resolution, we model how the respiratory complexes organize inside native mitochondria.
DOI: ads8738
Source: https://www.science.org/doi/10.1126/science.ads8738