上海科技大学物理科学与技术学院季泉江研究小组在研究中取得进展。他们揭示了几种已报道的Cas12f核酸酶系统反式活性比较†。相关论文于2025年3月18日发表在《中国化学》杂志上。

在本研究中，课题组研究人员对它们所表征的Cas12f蛋白的反式裂解活性进行了全面比较，发现它们都具有由双链DNA （dsDNA）、单链DNA （ssDNA）和单链RNA （ssRNA）触发的反式DNA酶活性。此外，研究小组在这些蛋白质中发现了对反式切割底物的不同碱基偏好。他们对Cas12f活性的进一步研究揭示了在不同条件下顺式和反式切割活性之间的复杂关系。他们的研究提供了Cas12f核酸酶反式切割特征的多方面表征，为更深入地理解Cas12f功能的机制提供了新的途径。

据悉，Cas12f同时具有顺式和反式切割活性，前者因其在基因组编辑中的应用而被广泛研究，而后者的探索较少，特别是用于诊断目的，并且主要集中在Un1Cas12f1上。

附：英文原文

Title: Systematic trans-Activity Comparison of Several Reported Cas12f Nucleases†

Author: Yannan Wang, Na Tang, Quanjiang Ji

Issue&Volume: 2025-03-18

Abstract: Cas12f possesses both cis- and trans-cleavage activities, with the former being extensively studied for its application in genome editing, while the latter remains less explored, particularly for diagnostic purposes, and is mostly focused on Un1Cas12f1. In this study, we conducted a comprehensive comparison of the trans-cleavage activities of four characterized Cas12f proteins, demonstrating that all four exhibit trans-DNase activity triggered by double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and single-stranded RNA (ssRNA). Additionally, we identified distinct base preferences for trans-cleavage substrates among these proteins. Our further investigation into the activities of Cas12f revealed the intricate relationship between cis- and trans-cleavage activities under various conditions. Our study provides a multifaceted characterization of the trans-cleavage features of Cas12f nucleases, offering new avenues for a deeper comprehension of the mechanisms underlying Cas12f's functionality.

DOI: 10.1002/cjoc.202401325

Source: https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202401325