近日，武汉大学化学与分子科学学院教授宋恒及其研究组报道了极性敏感和H₂O₂/脂滴序列激活的不对称菁氨酸探针实现动脉粥样硬化的多标记成像。这一研究成果发表在2025年3月17日出版的国际学术期刊《中国化学》上。

在这项研究中，研究团队提出了一种多色成像探针Cy7P-B极性，H₂O₂和脂滴来评估体内动脉粥样硬化斑块。Cy7P-B对环境极性敏感，可通过近红外比值监测极性变化。

此外，Cy7P-B具有H₂O₂/脂滴双分析物顺序活化特性。基于Cy7P-B的多功能特性，在动脉粥样硬化斑块中有效检测到动脉粥样硬化斑块、脂质积累和氧化应激上调等经典生物标志物，更重要的是首次检测到动脉粥样硬化中主动脉极性的变化。本研究为AS多物种成像提供了一种通用的分子设计方法，有助于有效的心血管疾病管理。

研究人员表示，动脉粥样硬化是一种脂蛋白驱动的疾病。由于动脉粥样硬化斑块形成的不可逆性，深入了解病理和准确识别在临床评估和治疗中尤为重要。动脉粥样硬化不仅伴随着脂滴积聚，而且与炎症密切相关，炎症伴随着活性氧（ROS）过多和微环境的变化。然而，目前仍缺乏一种简单、快速的检测平台，能够同时评估动脉粥样硬化多通道的多个指标。

附：英文原文

Title: Polarity Sensitive and H2O2/Lipid Droplets Sequence-Activated Asymmetric Cyanine Probe Achieves Multi-marker Imaging of Atherosclerosis

Author: Weihua Wang, Han Zhu, Bo Gu, Yongze Xie, Xinlei Xu, Shu-Lin Liu, Heng Song

Issue&Volume: 2025-03-17

Abstract: Atherosclerosis is a lipoprotein-driven disease. In-depth understanding of pathology and accurate identification are particularly important in clinical assessment and treatment due to the irreversibility of atherosclerotic plaque formation. Atherosclerosis is not only accompanied by lipid droplets accumulation but also closely related to inflammation, which is accompanied by excessive reactive oxygen species (ROS) and changes in microenvironment. However, there is still a lack of a simple and rapid detection platform to simultaneously evaluate multiple indicators of atherosclerosis in multiple channels. In this study, we propose a multicolor imaging probe Cy7P-B for polarity, H2O2 and lipid droplets to evaluate atherosclerotic plaques in vivo. Cy7P-B is sensitive to environmental polarity and can monitor polarity changes by near-infrared ratio. Moreover, Cy7P-B has H2O2/lipid droplets dual-analyte sequential activation characteristics. Based on the multifunctional properties of Cy7P-B, the classical biomarkers of atherosclerotic plaque, lipid accumulation and up-regulation of oxidative stress are effectively detected in atherosclerotic plaques, and more importantly, the change of aortic polarity in atherosclerosis was detected for the first time. This work provides a general molecular design approach for multi-species imaging of AS, which is helpful for effective cardiovascular disease stewardship.

DOI: 10.1002/cjoc.202401274

Source: https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202401274