3D人类参考图谱的构建和使用，这一成果由印第安纳大学Bruce W. Herr II研究组经过不懈努力而取得。2025年3月13日出版的《自然—方法学》发表了这项成果。

人类生物分子图谱计划（HuBMAP）旨在构建健康成人身体的三维人类参考图谱（HRA）。来自20多个联盟的专家合作开发了一个通用坐标框架（CCF）、知识图谱和工具，用于描述人体的多尺度结构（从器官和组织到细胞、基因和生物标志物），并以HRA为主题，描述衰老、疾病和其他扰动所发生的变化。HRA v.2.0涵盖了来自33个ASCT+B表和65个与本体相关的3D参考对象的4,499个独特解剖结构，1,195个细胞类型和2,089个生物标志物（如基因，蛋白质和脂质）。新的实验数据可以映射到HRA主题(1)细胞类型注释工具（例如Azimuth），(2)验证抗体面板或(3)通过空间注册组织数据。本文描述了HRA主题故事、术语、数据格式、本体验证、统一分析工作流、主题接口、教学材料、应用程序编程接口、灵活的混合云基础设施，并预览了图集主题应用。

附：英文原文

Title: Human BioMolecular Atlas Program (HuBMAP): 3D Human Reference Atlas construction and usage

Author: Brner, Katy, Blood, Philip D., Silverstein, Jonathan C., Ruffalo, Matthew, Satija, Rahul, Teichmann, Sarah A., Pryhuber, Gloria J., Misra, Ravi S., Purkerson, Jeffrey M., Fan, Jean, Hickey, John W., Molla, Gesmira, Xu, Chuan, Zhang, Yun, Weber, Griffin M., Jain, Yashvardhan, Qaurooni, Danial, Kong, Yongxin, Bueckle, Andreas, Herr, Bruce W.

Issue&Volume: 2025-03-13

Abstract: The Human BioMolecular Atlas Program (HuBMAP) aims to construct a 3D Human Reference Atlas (HRA) of the healthy adult body. Experts from 20+ consortia collaborate to develop a Common Coordinate Framework (CCF), knowledge graphs and tools that describe the multiscale structure of the human body (from organs and tissues down to cells, genes and biomarkers) and to use the HRA to characterize changes that occur with aging, disease and other perturbations. HRA v.2.0 covers 4,499 unique anatomical structures, 1,195 cell types and 2,089 biomarkers (such as genes, proteins and lipids) from 33 ASCT+B tables and 65 3D Reference Objects linked to ontologies. New experimental data can be mapped into the HRA using (1) cell type annotation tools (for example, Azimuth), (2) validated antibody panels or (3) by registering tissue data spatially. This paper describes HRA user stories, terminology, data formats, ontology validation, unified analysis workflows, user interfaces, instructional materials, application programming interfaces, flexible hybrid cloud infrastructure and previews atlas usage applications.

DOI: 10.1038/s41592-024-02563-5

Source: https://www.nature.com/articles/s41592-024-02563-5