波尔多大学Yohann Boutté研究小组报道了拟南芥通过动态中间顺式-高尔基管网络的ER-to-Golgi贩运。相关论文于2025年2月25日发表在《自然-细胞生物学》杂志上。

在这里，该课题组发现了一个网状的小管-囊泡网络，由MEMBRIN蛋白标记，主要独立于高尔基体，在ER-高尔基界面高度动态，并被ER诱导释放的腔内货物交叉。研究小组发现植物ERGICs通过与预先存在的高尔基体相互作用而变得稳定，并逐渐成熟为高尔基池，这一过程依赖于C24神经酰胺鞘脂。他们的研究是对高尔基体理解的一个重大转折，因为它确定了植物中的ERGICs包括一个与高尔基体无关的、高度动态的管状网络，从中产生了更稳定的高尔基体相关的前池结构。

据了解，内质网到高尔基体的转运是真核细胞分泌系统的一个核心过程，它保证了蛋白质和脂质的时空分选。然而，ER-高尔基中间区室（ERGICs）的性质和介导ERGICs与高尔基间过渡的分子机制，以及这些过程在真核生物中的普遍性，仍未被发现。

附：英文原文

Title: ER-to-Golgi trafficking through a dynamic intermediate cis-Golgi tubular network in Arabidopsis

Author: Fougre, Louise, Grison, Magali, Laquel, Patricia, Montrazi, Matheus, Cordelires, Fabrice, Fernndez-Monreal, Mnica, Poujol, Christel, Uemura, Tomohiro, Nakano, Akihiko, Ito, Yoko, Boutt, Yohann

Issue&Volume: 2025-02-25

Abstract: Endoplasmic reticulum (ER)-to-Golgi trafficking is a central process of the secretory system of eukaryotic cells that ensures proper spatiotemporal sorting of proteins and lipids. However, the nature of the ER–Golgi intermediate compartments (ERGICs) and the molecular mechanisms mediating the transition between ERGICs and the Golgi, as well as the universality of these processes among eukaryotes, remain undiscovered. Here we identify a reticulated tubulo-vesicular network, labelled by MEMBRIN proteins, that is mostly independent of the Golgi, highly dynamic at the ER–Golgi interface and crossed by ER-induced released luminal cargos. We find that plant ERGICs become stabilized by the interaction they establish with pre-existing Golgi and gradually mature into Golgi cisternae, this process being dependent on C24-ceramide sphingolipids. Our study is a major twist in the understanding of the Golgi, as it identifies that the ERGICs in plants comprise a Golgi-independent and highly dynamic tubular network from which arise more stable Golgi-associated pre-cisternae structures.

DOI: 10.1038/s41556-025-01624-x

Source: https://www.nature.com/articles/s41556-025-01624-x