美国哈佛大学Sheng Hui小组的一项最新研究提出了小鼠机体水平能量代谢定量通量模型。该研究于2025年2月20日发表于国际一流学术期刊《细胞—代谢》杂志上。

通过整合同位素示踪剂、质谱和同位素气体分析仪测量，小组建立了一个框架，系统地量化小鼠体内10种主要循环能量营养素在这些代谢过程中的通量，从而建立了一个生物体水平的能量代谢定量通量模型。该模型在野生型小鼠中揭示了循环营养素的代谢循环通量高于氧化通量，并且单个循环营养素的循环和氧化之间存在明显的分区。该框架在肥胖小鼠模型中的应用显示，在每只动物或每只瘦体重的基础上，ob/ob小鼠的代谢循环通量广泛升高，但在饮食诱导的肥胖小鼠中没有。它们的框架是揭示生理和疾病条件下能量代谢新特征的有价值的工具。

据悉，哺乳动物的组织以血液循环中的营养物质为食。在生物体水平上，哺乳动物的能量代谢包括循环营养物质的氧化、储存、相互转化和释放。

附：英文原文

Title: An organism-level quantitative flux model of energy metabolism in mice

Author: Bo Yuan, Will Doxsey, zlem Tok, Young-Yon Kwon, Yanshan Liang, Karen E. Inouye, Gkhan S. Hotamlgil, Sheng Hui

Issue&Volume: 2025-02-20

Abstract: Mammalian tissues feed on nutrients in the blood circulation. At the organism level, mammalian energy metabolism is comprised of the oxidation, storage, interconversion, and release of circulating nutrients. Here, by integrating isotope tracer infusion, mass spectrometry, and isotope gas analyzer measurement, we developed a framework to systematically quantify fluxes through these metabolic processes for 10 major circulating energy nutrients in mice, resulting in an organism-level quantitative flux model of energy metabolism. This model revealed in wild-type mice that circulating nutrients have metabolic cycling fluxes dominant to their oxidation fluxes, with distinct partitions between cycling and oxidation for individual circulating nutrients. Applications of this framework in obese mouse models showed extensive elevation of metabolic cycling fluxes in ob/ob mice but not in diet-induced obese mice on a per-animal or per-lean mass basis. Our framework is a valuable tool to reveal new features of energy metabolism in physiological and disease conditions.

DOI: 10.1016/j.cmet.2025.01.008

Source: https://www.cell.com/cell-metabolism/abstract/S1550-4131(25)00008-7