湖南师范大学化学化工学院熊二虎研究小组揭示了基于乙基键的稳健肽功能化金纳米颗粒高保真生物分析应用。2025年2月19日出版的《德国应用化学》杂志发表了这项成果。

在此，课题组提出了一种快速构建以Au-C≡C键为主题的肽功能化AuNP偶联物（PFCs）的方法，该方法可以在两分钟内完成。所得的Au-C≡C - PFCs比相应的Au-S PFCs表现出更好的稳定性和对生物硫醇的抗性，并且在高盐浓度、大pH值范围和不同温度下也表现出优异的稳定性。通过分子动力学模拟和x射线光电子能谱（XPS）证实了Au-C≡C共轭的机理。在细胞内半胱天冬酶成像实验中，Au-C≡C - pfc显著提高了信号保真度。总的来说，开发的策略为构建AuNP纳米探针提供了一种有前途的方法，允许可靠的检测并扩大了多种生物学应用的潜力。

研究人员表示，虽然Au-S键已广泛应用于制备金纳米颗粒（AuNP）生物偶联物，用于生物传感、细胞成像和生物医学研究，但复杂生物环境中的生物硫醇会因配体交换而严重干扰偶联物的稳定性。

附：英文原文

Title: Robust Peptide-Functionalized Gold Nanoparticles via Ethynyl Bonding for High-Fidelity Bioanalytical Applications

Author: Jinlian Du, Haili Xu, Xinyue Zhu, Keyu Long, Jiaqi Lang, Ling Jiang, Erhu Xiong, Ronghua Yang, Juewen Liu

Issue&Volume: 2025-02-19

Abstract: While Au-S bonds have been widely applied in preparing gold nanoparticle (AuNP) bioconjugates for biosensing, cell imaging, and biomedical research, biothiols in complex biological environments can seriously interfere with the stability of the conjugates due to ligand exchange. Herein, we communicate a robust and fast strategy for constructing peptide-functionalized AuNP conjugates (PFCs) using the Au-C≡C bond, which can be completed within two minutes. The resulting Au-C≡C PFCs exhibited better stability and resistance to biothiols than the corresponding Au-S PFCs, and also demonstrated excellent stability in high salt concentration, a wide range of pH values, and varying temperatures. The mechanism of Au-C≡C conjugation was confirmed using molecular dynamics simulation and X-ray photoelectron spectroscopy (XPS). The Au-C≡C PFCs significantly improved the signal fidelity in an intracellular caspase imaging assay. Overall, the developed strategy provides a promising approach for constructing AuNP nanoprobes, allowing reliable detection and broadening the potential for diverse biological applications.

DOI: 10.1002/anie.202424351

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202424351