2025年12月2日出版的《细胞》杂志发表了科学家的一项最新成果。来自上海交通大学的林尤舜课题组的研究开发出了植物热感知的逐步解码机制将脂质重塑与核信号级联联系起来。
小组证明了二酰基甘油激酶7 （DGK7）在质膜上响应热应激，将二酰基甘油转化为第二信使磷脂酸（PA）。随后，金属依赖性磷酸二酯酶（MdPDE1）感知PA，通过与PA结合获得活性，并易位到核细胞上降解另一个第二信使环磷酸腺苷（cAMP）。然后MdPDE1通过改变cAMP信号引发转录景观变化。
此外，G蛋白亚基耐热性2 （TT2）通过Ser477去磷酸化抑制DGK7活性，阻断MdPDE1活性和核易位。值得注意的是，田间试验证明了这一机制的应用前景，根据需要赋予水稻不同程度的耐热性。这项研究建立了一个完整的分层热解码机制，为创造耐热作物开辟了机会，有助于减轻全球变暖造成的产量损失。
据悉，热应激触发细胞膜脂质重塑，但这是否向植物发出了感知高温的信号，以及这些物理信号如何被解码为生物信号，目前尚不清楚。
附：英文原文
Title: A stepwise decoding mechanism for heat sensing in plants connects lipid remodeling to a nuclear signaling cascade
Author: Yi Kan, Xiao-Rui Mu, Fangfang Qu, Zhi-An Dai, Jin Gao, Ning-Jing Liu, Siqi Li, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Xiaolan Huang, Yi-Bing Yang, Shuang-Qin Guo, Jie-Jie Lei, Ying-Jie Cao, Ji-Fu Zhou, Pengcheng Li, Jianchuan Wang, Yixue Li, Hong-Xuan Lin, Youshun Lin
Issue&Volume: 2025-12-02
Abstract: Heat stress triggers cell membrane lipid remodeling, yet whether this signals plants to perceive high temperatures and how such physical signals are decoded into biological signals remains unclear. Here, we demonstrate that diacylglycerol kinase 7 (DGK7) responds to heat stress at the plasma membrane, converting diacylglycerol into the second messenger, phosphatidic acid (PA). Subsequently, metal-dependent phosphodiesterase (MdPDE1) senses PA, acquires its activity by binding to PA, and translocates to the nucleus to degrade another second messenger, cyclic adenosine monophosphate (cAMP). MdPDE1 then elicits transcriptional landscape changes via altering cAMP signaling. Furthermore, G protein subunit thermotolerance 2 (TT2) inhibits DGK7 activity by Ser477 dephosphorylation, blocking MdPDE1 activity and nuclear translocation. Notably, field trials demonstrated the promising applications of this mechanism that confers varying degrees of rice thermotolerance as needed. This study establishes a complete hierarchical thermo-decoding mechanism that opens opportunities for creating customized heat-tolerant crops, aiding in mitigating yield losses from global warming.
DOI: 10.1016/j.cell.2025.11.003
Source: https://www.cell.com/cell/abstract/S0092-8674(25)01252-8