中国科学院动物研究所于乐谦与合作团队发现用于研究人体植入和植入失败的3D体外模型。这一研究成果于2025年12月23日发表在国际顶尖学术期刊《细胞》上。
为了克服这个问题，研究小组开发了一种3D芯片内植入模型，主题是人类囊胚或囊胚与生物工程人类子宫内膜组织共同培养，称为子宫内膜样。该系统成功地概括了人类植入和早期植入后发展的关键事件。重要的是，当对来自RIF患者的植入主题样本进行建模时，课题组观察到与来自生育对照组的子宫内膜样体相比，囊胚植入能力明显降低。
此外，美国食品和药物管理局（FDA）批准的化合物的靶向筛选确定了显著提高RIF源性子宫内膜样物植入效率的候选化合物。总之，这个3D平台能够对人类植入和植入失败进行机制研究，并提供了一种可扩展的方法来评估临床环境中改善胚胎-子宫内膜相互作用的治疗策略。
据介绍，更好地了解人类植入对于改善辅助生殖结果和解决复发性植入失败（RIF）至关重要。然而，伦理约束和对人类胚胎的有限获取使得直接研究具有挑战性。
附：英文原文
Title: A 3D in vitro model for studying human implantation and implantation failure
Author: Qian Li, Yang Yuan, Wentao Zhao, Yuanjun Li, Juanzi Shi, Yu Xiu, Mi Han, Yan Han, Junmei Zhang, Shuhan Cheng, Xin Qi, Xizhuang Sun, Tan Jia, Jiaqi Xing, Siwei Deng, Xiaodi Yan, Seiya Oura, Hongfei Li, Ying Sun, Huiyao Yuan, Xiaohong Ma, Miaomiao Xin, Jianchao Zhao, Xili Zhu, Cong Wang, Qin Wang, Ge Lin, Xiaokui Yang, Yulei Wei, Jun Wu, Hongmei Wang, Leqian Yu
Issue&Volume: 2025-12-23
Abstract: A better understanding of human implantation is essential for improving assisted reproduction outcomes and addressing recurrent implantation failure (RIF). However, ethical constraints and limited access to human embryos make direct studies challenging. To overcome this, we developed a 3D in-chip implantation model using human blastoids or blastocysts co-cultured with a bioengineered human endometrial tissue, termed endometrioid. This system successfully recapitulates key events of human implantation and early post-implantation development. Importantly, when modeling implantation using samples derived from RIF patients, we observed significantly reduced blastoid implantation capability compared with endometrioids from fertile controls. Furthermore, a targeted screen of U.S. Food and Drug Administration (FDA)-approved compounds identified candidates that markedly enhanced implantation efficiency in RIF-derived endometrioids. Together, this 3D platform enables mechanistic investigation of human implantation and implantation failure and offers a scalable approach to evaluate therapeutic strategies for improving embryo-endometrium interaction in a clinical setting.
DOI: 10.1016/j.cell.2025.10.026
Source: https://www.cell.com/cell/abstract/S0092-8674(25)01230-9