英国Babraham研究所Peter J. Rugg-Gunn小组近日取得一项新成果。经过不懈努力，他们的研究认为体外模拟人类胚胎植入。相关论文于2025年12月23日发表于国际顶尖学术期刊《细胞》杂志上。

由于难以获得植入胚胎和缺乏合适的模型系统，该课题组人员对这些阶段的了解有限。研究组建立了一个体外模型，再现了人类接受子宫内膜浅层的腔室、腺室和间质室。人类胚胎和囊胚植入子宫内膜模型，实现植入后的特征，包括胎盘发育早期事件基础的高级滋养细胞结构。第14天胚胎-子宫内膜界面的单细胞RNA测序揭示了胚胎和子宫内膜之间预测的分子相互作用。破坏细胞外滋养层细胞和子宫内膜间质细胞之间的信号相互作用，在滋养层细胞生长过程中形成缺陷，证明了串扰过程对维持胚胎发生的重要性。这个平台为研究人类胚胎植入的早期阶段提供了机会。

研究人员表示，将人类胚胎植入子宫内膜是妊娠期的一个关键事件，因为它标志着怀孕的开始，而且容易出现高失败率。

附：英文原文

Title: Modeling human embryo implantation in vitro

Author: Matteo A. Molè, Sarah Elderkin, Irene Zorzan, Christopher Penfold, Nicole Horsley, Alexandra Pokhilko, Max Polanek, Andrea Palomar, Molika Sinha, Yang Wang, Alicia Quionero, Charalampos Androulidakis, Richard Acton, Kathryn Balmanno, Anneliese Jarman, Jhanavi Srinivasan, Adam Bendall, Sara Morales-álvarez, Roberto Yagüe-Serrano, Katie Heywood, Stephen Harbottle, Mina Vasilic, Suzanne Cawood, Srividya Seshadri, Paul Serhal, Lauren Weavers, Ippokratis Sarris, Anastasia Mania, Rachel Gibbons, Lucy Laurier, Immaculada Sánchez-Ribas, Amparo Mercader, Pilar Alamá, Anthony Hoa Bui, Graham J. Burton, Tereza Cindrova-Davies, Ridma C. Fernando, Afshan McCarthy, Lusine Aghajanova, Liesl Nel-Themaat, Ruth B. Lathi, Simon J. Cook, Kathy K. Niakan, Alexander R. Dunn, Francisco Domínguez, Peter J. Rugg-Gunn

Issue&Volume: 2025-12-23

Abstract: Implantation of a human embryo into the endometrium is a crucial event in gestation, as it marks the initiation of a pregnancy and is prone to high failure rates. We have limited understanding of these stages because of the inaccessibility of implanting embryos and the lack of suitable model systems. Here, we establish an in vitro model that recapitulates the luminal, glandular, and stromal compartments of the superficial layer of receptive human endometrium. Human embryos and blastoids implant into the endometrial model, achieving post-implantation hallmarks including advanced trophoblast structures that underlie early events in placental development. Single-cell RNA sequencing of the embryo-endometrial interface at day 14 uncovers predicted molecular interactions between conceptus and endometrium. Disrupting signaling interactions between extravillous trophoblast and endometrial stromal cells caused defects in trophoblast outgrowth, demonstrating the importance of crosstalk processes to sustain embryogenesis. This platform opens the opportunity to investigate early stages of human embryo implantation.

DOI: 10.1016/j.cell.2025.10.027

Source: https://www.cell.com/cell/abstract/S0092-8674(25)01232-2