在这里，该研究团队提出了一种基于半透性胶囊（SPCs）的多功能技术，专门用于各种高通量核酸测定，包括单细胞基因组和mRNA测序，以及基于感兴趣的核酸标记物的基于荧光激活细胞分选的个体转录组分离。由于具有生物相容性，SPCs支持长时间的单细胞培养和克隆扩增，从而克服了液滴微流体平台的基本限制。总体而言，SPCs代表了可自组化和广泛适用的工具，用于易于主题，可扩展的单细胞组学应用，建立在多步骤生化反应上。
据悉，生物系统本质上是复杂和异构的。破译这种复杂性越来越依赖于高通量单细胞组学方法和工具，有效地探测细胞表型和基因型。
附：英文原文
Title: High-throughput single cell omics using semipermeable capsules
Author: Denis Baronas, Simonas Norvaisis, Justina Zvirblyte, Greta Leonaviciene, Vincenta Mikulenaite, Karolis Goda, Vytautas Kaseta, Karolis Sablauskas, Laimonas Griskevicius, Simonas Juzenas, Linas Mazutis
Issue&Volume: 2025-12-18
Abstract: Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput single-cell omics methods and tools that efficiently probe the cellular phenotype and genotype. Here we present a versatile technology based on semipermeable capsules (SPCs), tailored for a variety of high-throughput nucleic acid assays, including single-cell genome and mRNA sequencing, and fluorescence-activated cell sorting-based isolation of individual transcriptomes based on nucleic acid marker of interest. Being biocompatible, the SPCs support single-cell cultivation and clonal expansion over long periods of time thereby overcoming a fundamental limitation of droplet microfluidics platforms. Overall, the SPCs represent customizable and broadly applicable tool for easy-to-use, scalable single-cell omics applications that are built on multi-step biochemical reactions.
DOI: ady7227
Source: https://www.science.org/doi/10.1126/science.ady7227