近日，北京大学王静团队研究了长效膜蛋白标记交联适体的进化。2025年12月15日，《美国化学会志》发表了这一成果。

随着膜蛋白研究需求的不断增长，再加上适体作为配体候选体的结构灵活性和高通量筛选效率，使适体成为研究膜蛋白的重要工具。

在这项工作中，研究组提出了一种新的体外进化策略，以开发共价适体，利用氟硫交换（SuFEx）化学原理。这种策略有助于筛选对靶膜蛋白具有高特异性的适体，最终产生交联适体（xl-aptamers）。这些适体对其靶蛋白表现出稳定和特异性的结合亲和力，从而实现有效的细胞分类。它们的性能强调了在单细胞和空间蛋白质组学研究中的巨大应用潜力。

附：英文原文

Title: Evolution of Cross-Linking Aptamers for Long-Lasting Membrane Protein Labeling

Author: Siqi Bian, Qianwei Qiu, Chenshuo Zhang, Haiyan Cao, Hong Xuan, Xueling Yan, Jun Li, Wenlong Chen, Xiyang Liu, Yifan Chen, Jinchen Long, Huang Su, Jing Wang, Liqin Zhang

Issue&Volume: December 15, 2025

Abstract: The growing demand for membrane protein research, coupled with the structural flexibility and high-throughput screening efficiency of aptamers as ligand candidates, has established aptamers as a robust tool for investigating membrane proteins. In this study, we present a novel in vitro evolution strategy for the development of covalent aptamers, leveraging the principles of fluorine–sulfur exchange (SuFEx) chemistry. This strategy facilitates the screening of aptamers with high specificity for target membrane proteins, ultimately yielding cross-linking aptamers (xl-aptamers). These aptamers exhibit sustained and specific binding affinity toward their target proteins, thereby enabling efficient cell classification. Their performance underscores considerable potential for applications in single-cell and spatial proteomics research.

DOI: 10.1021/jacs.5c16570

Source: https://pubs.acs.org/doi/abs/10.1021/jacs.5c16570