ARF3介导的生长素信号在黄瓜性别决定中起重要作用，这一成果由
研究人员发现了一个在黄瓜心皮发育中起重要作用的生长素反应因子3 （CsARF3）。CsARF3基因敲除导致雌花数量增加，而CsARF3基因过表达导致雌花数量增加。CsARF3通过直接刺激分生组织维持基因CsSTM的表达，抑制雌核细胞基因CsWIP1的活性，促进雌性化。生长素和乙烯在性别决定过程中表现出互惠关系，乙烯在花发育早期通过生长素促进心皮形成。然后，心皮中的生长素信号传导促进乙烯生物合成，抑制雄蕊发育。
研究人员表示，性别决定是雌雄花形成的基础，在植物育种中具有特别重要的意义。生长素和乙烯调节瓜类的雌性。
附：英文原文
Title: ARF3-mediated auxin signaling is essential for sex determination in cucumber
Author: Lijie Han, Min Li, Chuang Li, Bosi Zhao, Zhongyi Wang, Ye Liu, Zhihan Liu, Yafei Huang, Liu Liu, Haoran Geng, Yuting He, Jingyu Guo, Shaoyun Wang, Liming Wang, Chaoheng Gu, Junjun Shen, Zheng Li, Jianyu Zhao, Zhaoyang Zhou, Xiaolan Zhang
Issue&Volume: 2025-12-11
Abstract: Sex determination underpins genesis of male and female flowers with particularly important implications in plant breeding. Auxin and ethylene regulate femaleness in cucurbits. Here, we identified an auxin response factor 3 (CsARF3) that plays an essential role in carpel development in cucumber. Knockout of CsARF3 resulted in an androecious phenotype with only male flowers, whereas overexpression of CsARF3 led to increased number of female flowers. CsARF3 promotes femaleness by directly stimulating the expression of meristem maintenance gene CsSTM, and repressing the activity of gynoecious gene CsWIP1. Auxin and ethylene exhibit a reciprocal relationship during sex determination, in which ethylene promotes carpel formation through auxin at the early stage of flower development. The auxin signaling in carpels then enhances ethylene biosynthesis to inhibit stamen development.
DOI: adv2006
Source: https://www.science.org/doi/10.1126/science.adv2006