近日，美国加州大学吉娜及其研究小组提出了FACED 2.0可实现体内大规模电压和钙成像。该研究于2025年12月9日发表于国际一流学术期刊《自然—方法学》杂志上。

在这里，课题组研究人员优化了一种千赫兹帧率双光子荧光显微镜，具有全光兆赫线扫描速率，以实现亚细胞分辨率的大面积和体积的超快成像。将该技术应用于活体电压和钙成像，该课题组人员展示了同时记录200多个神经元的电压活动和14000多个神经元的钙活动，以及幼体斑马鱼大脑的体积钙成像。

据悉，在大尺度和高时空分辨率下监测神经元活动对于理解大脑内部的信息处理至关重要。

附：英文原文

Title: FACED 2.0 enables large-scale voltage and calcium imaging in vivo

Author: Zhong, Jian, Natan, Ryan G., Zhang, Qinrong, Wong, Justin S. J., Miehl, Christoph, Bose, Krishnashish, Lu, Xiaoyu, St-Pierre, Franois, Guo, Su, Doiron, Brent, Tsia, Kevin K., Ji, Na

Issue&Volume: 2025-12-09

Abstract: Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. Here we optimized a kilohertz-frame-rate two-photon fluorescence microscope with an all-optical megahertz line-scan rate to achieve ultrafast imaging across large areas and volumes at subcellular resolution. Applying this technique to in vivo voltage and calcium imaging, we demonstrated simultaneous recording of voltage activity over 200 neurons and calcium activity over 14,000 neurons from the mouse visual cortex, as well as volumetric calcium imaging of the larval zebrafish brain.

DOI: 10.1038/s41592-025-02925-7

Source: https://www.nature.com/articles/s41592-025-02925-7