苏黎世大学Bernd Bodenmiller课题组近日取得一项新成果。经过不懈努力，他们报道了高分辨率成像质量细胞术绘制亚细胞结构。相关论文发表在2025年10月30日出版的《自然—方法学》杂志上。

本文报道了一种提高IMC分辨率的方法，使其接近光学显微镜的分辨率。高分辨率IMC （HR-IMC）采用过采样方法与基于点扩散函数的反卷积相结合，以实现低于350nm的分辨率。小组展示了HR-IMC在解析亚细胞结构（如核病灶和线粒体网络）方面的性能，这些亚细胞结构以前是IMC无法检测到的，并将其应用于可视化化疗诱导的患者源性卵巢癌细胞的扰动。HR-IMC将高度多元的IMC分析扩展到亚细胞状态，使分析细胞生物学特征和疾病特征成为可能。

研究人员表示，成像质细胞术（IMC）是一种功能强大的多路成像技术，旨在研究健康和疾病中组织的细胞表型和空间组织。IMC的空间分辨率目前为1μm，可以分辨单细胞和大的亚细胞区室，但不能分辨亚微米的细胞内结构。

附：英文原文

Title: High-resolution imaging mass cytometry to map subcellular structures

Author: Bollhagen, Alina, Whipman, James, Coelho, Ricardo, Heinzelmann-Schwarz, Viola, Jacob, Francis, Bodenmiller, Bernd

Issue&Volume: 2025-10-30

Abstract: Imaging mass cytometry (IMC) is a powerful multiplexed imaging technology used to investigate cell phenotypes and spatial organization of tissue in health and disease. The spatial resolution of IMC is presently at 1μm, enabling the resolution of single cells and large subcellular compartments but not submicrometer intracellular structures. Here we report a method to improve the resolution of IMC so that it approaches that of light microscopy. High-resolution IMC (HR-IMC) uses an oversampling approach coupled with point-spread function-based deconvolution to achieve a resolution below 350nm. We demonstrate the performance of HR-IMC in resolving subcellular structures, such as nuclear foci and mitochondrial networks previously undetectable with IMC, and applied it to visualize chemotherapy-induced perturbation of patient-derived ovarian cancer cells. HR-IMC extends highly multiplex IMC analyses into the subcellular regime, enabling analysis of cell biological features and characteristics of disease.

DOI: 10.1038/s41592-025-02889-8

Source: https://www.nature.com/articles/s41592-025-02889-8