洛克菲勒大学Nadav Shai研究小组在研究中取得进展。他们报道了埃及伊蚊成虫的单核转录图谱。2025年10月30日出版的《细胞》发表了这项成果。

该研究组展示了埃及伊蚊细胞图谱，这是一个全面的单核RNA测序数据集，来自19个成年雌性和雄性埃及伊蚊解剖组织的367,000多个细胞核，提供了蚊子生物学的细胞水平分辨率。该课题组发现了新的细胞类型，并扩大了他们对所有感觉组织中化学感受器的感觉神经元组织的理解。他们的分析揭示了天线和大脑中男性特有的细胞和两性二态基因的表达。在雌性蚊子身上，该课题组研究人员发现，在吸血后，大脑中发生最广泛的转录变化的是神经胶质细胞，而不是神经元。他们的发现为蚊子行为和两性异形的细胞基础提供了见解。埃及伊蚊蚊子细胞图谱抗性使得系统地研究所有蚊子组织中细胞类型特异性表达成为可能。

据了解，雌性埃及伊蚊捕食人类和传播病原体的非凡能力依赖于她独特的生物学。

附：英文原文

Title: A single-nucleus transcriptomic atlas of the adult Aedes aegypti mosquito

Author: Olivia V. Goldman, Alexandra E. DeFoe, Yanyan Qi, Yaoyu Jiao, Shih-Che Weng, Brittney Wick, Leah Houri-Zeevi, Priyanka Lakhiani, Takeshi Morita, Jacopo Razzauti, Adriana Rosas-Villegas, Yael N. Tsitohay, Madison M. Walker, Ben R. Hopkins, Joshua X.D. Ang, Igor Antoshechkin, Yu Cai, Fangying Chen, Yen-Chung Chen, Julien Devilliers, Linhan Dong, Roberto Feuda, Paolo Gabrieli, Artyom Kopp, Hyeogsun Kwon, Hsing-Han Li, Tzu-Chiao Lu, Thalita Lucio, Joo T. Marques, Marcus F. Oliveira, Roenick P. Olmo, Umberto Palatini, Zeaan M. Pithawala, Julien Pompon, Yan Reis, Joo Rodrigues, Ryan C. Smith, Maximilian Haeussler, Omar S. Akbari, Laura B. Duvall, Helen White-Cooper, Trevor R. Sorrells, Roshan Sharma, Hongjie Li, Leslie B. Vosshall, Nadav Shai

Issue&Volume: 2025-10-30

Abstract: The female Aedes aegypti mosquito’s remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Aedes aegypti Mosquito Cell Atlas, a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues of adult female and male Aedes aegypti, providing cellular-level resolution of mosquito biology. We identify novel cell types and expand our understanding of sensory neuron organization of chemoreceptors across all sensory tissues. Our analysis uncovers male-specific cells and sexually dimorphic gene expression in the antenna and brain. In female mosquitoes, we find that glial cells, rather than neurons, undergo the most extensive transcriptional changes in the brain following blood feeding. Our findings provide insights into the cellular basis of mosquito behavior and sexual dimorphism. The Aedes aegypti Mosquito Cell Atlas resource enables systematic investigation of cell-type-specific expression across all mosquito tissues.

DOI: 10.1016/j.cell.2025.10.008

Source: https://www.cell.com/cell/abstract/S0092-8674(25)01137-7