近日，中山大学李陈龙及其团队发现，组蛋白乙酰转移酶1与CONSTANS相互作用促进拟南芥开花。相关论文于2025年1月23日在线发表在《遗传学报》上。

研究人员阐明了组蛋白乙酰转移酶1（HAC1）通过在两个关键花期整合因子FT和SOC1处施加活性表观遗传标记，促进开花的关键功能，从而调控它们的表达以触发开花过程。研究人员显示，HAC1在体内外与CONSTANS（CO）发生物理相互作用。染色质免疫沉淀结果表明，HAC1直接结合到FT和SOC1基因位点。

缺失HAC1会损害CO介导的FT和SOC1转录激活，从而影响开花的促进作用。此外，CO突变导致HAC1在FT和SOC1上的富集减少，表明CO招募HAC1到FT和SOC1位点。最后，HAC1和CO都需要在FT和SOC1上提高组蛋白乙酰化水平。

总之，这些研究结果揭示了HAC1介导的组蛋白乙酰化通过CO依赖性的FT和SOC1激活促进开花。

据悉，组蛋白修饰，包括组蛋白乙酰化，在调控开花过程中发挥着至关重要的作用。CBP/p300家族的HAC1通过介导组蛋白乙酰化促进花器过渡的过程；然而，具体机制仍不完全明了。特别是，HAC1如何参与开花调控网络，以及HAC1在开花调控过程中直接作用的基因仍不清楚。

附：英文原文

Title: The HISTONE ACETYLTRANSFERASE 1 interacts with CONSTANS to promote flowering in Arabidopsis

Author: Chenlong Li

Issue&Volume: 2025/01/23

Abstract: Chromatin modifications including histone acetylation play essential roles in regulating flowering. The CBP/p300 family HISTONE ACETYLTRANSFERASE 1 (HAC1), which mediates histone acetylation, promotes the process of floral transition; however, the precise mechanism remains largely unclear. Specifically, how HAC1 is involved in the flowering regulatory network and which genes are the direct targets of HAC1 during flowering regulation are still unknown. In this study, we elucidated the critical function of HAC1 in promoting flowering via exerting active epigenetic markers at two key floral integrators, FT and SOC1, thereby regulating their expression to trigger the flowering process. We show that HAC1 physically interacts with CONSTANS (CO) in vivo and in vitro. Chromatin immunoprecipitation results indicate that HAC1 directly binds to the FT and SOC1 loci. Loss of HAC1 impairs CO-mediated transcriptional activation of FT and SOC1 in promoting flowering. Moreover, CO mutation leads to the decreased enrichment of HAC1 at FT and SOC1, indicating that CO recruits HAC1 to FT and SOC1. Finally, HAC1, as well as CO, is required for the elevated histone acetylation level at FT and SOC1. Taken together, our finding reveals that HAC1-mediated histone acetylation boots flowering via a CO-dependent activation of FT and SOC1.

DOI: 10.1016/j.jgg.2025.01.010

Source: https://www.sciencedirect.com/science/article/abs/pii/S1673852725000256