中国科学院脑科学与智能技术卓越创新中心刘赐融等研究人员，合作绘制出
研究人员获得了猕猴、狨猴和小鼠小脑的单细胞空间转录组图谱，并鉴定出了灵长类特有的细胞亚型，包括表现出不同的谷氨酸离子型受体Delta型亚基2（GRID2）基因表达的浦肯野细胞和分子层中间神经元。
所有物种的前部、后部和前庭区域均发现了不同的基因表达谱，而区域特异性的基因表达主要在灵长类动物的颗粒层和小鼠的普肯耶层中观察到。
小脑皮层中的基因表达梯度与清醒功能性磁共振成像所揭示的功能连接梯度非常吻合，在灵长类动物和小鼠之间的叶特异性差异大于两种灵长类动物之间的差异。这些全面的图谱和比较分析为理解小脑的演化和功能奠定了基础。
据了解，灵长类动物小脑的分子和细胞组织特征仍然不够明确。
附：英文原文
Title: Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex
Author: Shijie Hao, Xiaojia Zhu, Zhi Huang, Qianqian Yang, Hean Liu, Yan Wu, Yafeng Zhan, Yu Dong, Chao Li, He Wang, Elize Haasdijk, Zihan Wu, Shenglong Li, Haotian Yan, Lijing Zhu, Shiyong Guo, Zefang Wang, Aojun Ye, Youning Lin, Luman Cui, Xing Tan, Huanlin Liu, Mingli Wang, Jing Chen, Yanqing Zhong, Wensi Du, Guangling Wang, Tingting Lai, Mengdi Cao, Tao Yang, Yuanfang Xu, Ling Li, Qian Yu, Zhenkun Zhuang, Ying Xia, Ying Lei, Yingjie An, Mengnan Cheng, Yun Zhao, Lei Han, Yue Yuan, Xinxiang Song, Yumo Song, Liqin Gu, Chang Liu, Xiumei Lin, Ruiqi Wang, Zhifeng Wang, Yang Wang, Shenyu Li, Huanhuan Li, Jingjing Song, Mengni Chen, Wanqiu Zhou, Nini Yuan, Suhong Sun, Shiwen Wang, Yu Chen, Mingyuan Zheng, Jiao Fang, Ruiyi Zhang, Shuzhen Zhang, Qinwen Chai, Jiabing Liu, Wu Wei, Jie He, Haibo Zhou, Yangang Sun, Zhen Liu, Chuanyu Liu, Jianhua Yao, Zhifeng Liang, Xun Xu, Muming Poo, Chengyu Li, Chris I. De Zeeuw, Zhiming Shen, Zhiyong Liu, Longqi Liu, Shiping Liu, Yidi Sun, Cirong Liu
Issue&Volume: 2024-09-27
Abstract: The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases of macaque, marmoset, and mouse cerebella and identified primate-specific cell subtypes, including Purkinje cells and molecular-layer interneurons, that show different expression of the glutamate ionotropic receptor Delta type subunit 2 (GRID2) gene. Distinct gene expression profiles were found in anterior, posterior, and vestibular regions in all species, whereas region-selective gene expression was predominantly observed in the granular layer of primates and in the Purkinje layer of mice. Gene expression gradients in the cerebellar cortex matched well with functional connectivity gradients revealed with awake functional magnetic resonance imaging, with more lobule-specific differences between primates and mice than between two primate species. These comprehensive atlases and comparative analyses provide the basis for understanding cerebellar evolution and function.
DOI: ado3927
Source: https://www.science.org/doi/10.1126/science.ado3927