中国科学院遗传与发育生物学研究所何康敏团队近期取得重要工作进展，他们研究提出，与网格蛋白相关的载体通过“kiss-and-run”机制实现再循环。相关研究成果2024年9月19日在线发表于《自然—细胞生物学》杂志上。

据介绍，内吞作用和再循环控制着所有真核细胞中各种物质的摄取和回收，包括膜蛋白和脂质。这些过程对细胞生长、组织、功能和环境交流至关重要。然而，高效、快速的内吞循环机制仍然知之甚少。

通过利用生物传感器和基于成像的筛查，研究人员发现了一种将内吞作用和快速回收结合起来的回收机制，研究人员将其命名为网格蛋白相关的快速内体回收途径（CARP）。含有网格蛋白、AP1、Arf1、Rab1和Rab11的网格蛋白相关小管泡载体在早期核内体的亚结构域产生，然后沿着肌动蛋白运输到细胞表面，但缺乏多聚体检索复合物。与网格蛋白相关的回收载体与质膜发生了部分融合。随后，它们被动力蛋白从膜上释放出来，重新进入细胞。多种受体利用并调节CARP，以实现内吞作用后的快速循环。

因此，CARP代表了一种以前未被认识的具有“kiss-and-run“膜融合的内吞再循环机制。

附：英文原文

Title: Clathrin-associated carriers enable recycling through a kiss-and-run mechanism

Author: Xu, Jiachao, Liang, Yu, Li, Nan, Dang, Song, Jiang, Amin, Liu, Yiqun, Guo, Yuting, Yang, Xiaoyu, Yuan, Yi, Zhang, Xinyi, Yang, Yaran, Du, Yongtao, Shi, Anbing, Liu, Xiaoyun, Li, Dong, He, Kangmin

Issue&Volume: 2024-09-19

Abstract: Endocytosis and recycling control the uptake and retrieval of various materials, including membrane proteins and lipids, in all eukaryotic cells. These processes are crucial for cell growth, organization, function and environmental communication. However, the mechanisms underlying efficient, fast endocytic recycling remain poorly understood. Here, by utilizing a biosensor and imaging-based screening, we uncover a recycling mechanism that couples endocytosis and fast recycling, which we name the clathrin-associated fast endosomal recycling pathway (CARP). Clathrin-associated tubulovesicular carriers containing clathrin, AP1, Arf1, Rab1 and Rab11, while lacking the multimeric retrieval complexes, are generated at subdomains of early endosomes and then transported along actin to cell surfaces. Unexpectedly, the clathrin-associated recycling carriers undergo partial fusion with the plasma membrane. Subsequently, they are released from the membrane by dynamin and re-enter cells. Multiple receptors utilize and modulate CARP for fast recycling following endocytosis. Thus, CARP represents a previously unrecognized endocytic recycling mechanism with kiss-and-run membrane fusion.

DOI: 10.1038/s41556-024-01499-4

Source: https://www.nature.com/articles/s41556-024-01499-4