美国哈佛大学等研究人员合作发现，类器官培养促进小鼠肌母细胞去分化为能够完全再生肌肉的干细胞。相关论文于2024年9月11日发表在《自然—生物技术》杂志上。

据了解，用于骨骼肌疾病的实验性细胞疗法迄今取得的成功有限，主要原因是使用了已定向的成肌祖细胞而非真正的肌肉干细胞（卫星细胞）。

附：英文原文

Title: Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration

Author: Price, Feodor D., Matyas, Mark N., Gehrke, Andrew R., Chen, William, Wolin, Erica A., Holton, Kristina M., Gibbs, Rebecca M., Lee, Alice, Singu, Pooja S., Sakakeeny, Jeffrey S., Poteracki, James M., Goune, Kelsey, Pfeiffer, Isabella T., Boswell, Sarah A., Sorger, Peter K., Srivastava, Mansi, Pfaff, Kathleen Lindahl, Gussoni, Emanuela, Buchanan, Sean M., Rubin, Lee L.

Issue&Volume: 2024-09-11

Abstract: Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they use committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro-derived satellite cells (idSCs) from skeletal muscle tissue. When transplanted in small numbers into mouse muscle, mouse idSCs fuse into myofibers, repopulate the satellite cell niche, self-renew, support multiple rounds of muscle regeneration and improve force production on par with freshly isolated satellite cells in damaged skeletal muscle. We compared the epigenomic and transcriptional signatures between idSCs, myoblasts and satellite cells and used these signatures to identify core signaling pathways and genes that confer idSC functionality. Finally, from human muscle biopsies, we successfully generated satellite cell-like cells in vitro. After further development, idSCs may provide a scalable source of cells for the treatment of genetic muscle disorders, trauma-induced muscle damage and age-related muscle weakness.

DOI: 10.1038/s41587-024-02344-7

Source: https://www.nature.com/articles/s41587-024-02344-7