通过在超过1800个人类卵母细胞中进行高分辨率成像，研究人员揭示了在纺锤体双极化过程中形成的多极中间体的典型状态，并阐明了这一过程的机制。研究人员发现，多极中间体的生成与多个动粒簇中形成的小极体有关。
研究人员进一步确定了HAUS6、KIF11和KIF18A在纺锤体双极化中的关键作用，并在卵母细胞或胚胎缺陷的不育患者中发现了这些基因的突变。这些结果为人类卵母细胞中纺锤体双极化的生理和病理机制提供了新见解。
研究人员表示，纺锤体双极化是指微管质转变为双极纺锤体的过程，这是精确染色体分离的前提。与有丝分裂细胞不同，人类卵母细胞中纺锤体双极化的过程和机制仍不清楚。
附：英文原文
Title: Mechanisms of minor pole–mediated spindle bipolarization in human oocytes
Author: Tianyu Wu, Yuxi Luo, Meiling Zhang, Biaobang Chen, Xingzhu Du, Hao Gu, Siyuan Xie, Zhiqi Pan, Ran Yu, Ruiqi Hai, Xiangli Niu, Guimin Hao, Liping Jin, Juanzi Shi, Xiaoxi Sun, Yanping Kuang, Wen Li, Qing Sang, Lei Wang
Issue&Volume: 2024-08-23
Abstract: Spindle bipolarization, the process of a microtubule mass transforming into a bipolar spindle, is a prerequisite for accurate chromosome segregation. In contrast to mitotic cells, the process and mechanism of spindle bipolarization in human oocytes remains unclear. Using high-resolution imaging in more than 1800 human oocytes, we revealed a typical state of multipolar intermediates that form during spindle bipolarization and elucidated the mechanism underlying this process. We found that the minor poles formed in multiple kinetochore clusters contribute to the generation of multipolar intermediates. We further determined the essential roles of HAUS6, KIF11, and KIF18A in spindle bipolarization and identified mutations in these genes in infertile patients characterized by oocyte or embryo defects. These results provide insights into the physiological and pathological mechanisms of spindle bipolarization in human oocytes.
DOI: ado1022
Source: https://www.science.org/doi/10.1126/science.ado1022