北京大学段小洁团队近期取得重要工作进展，他们研究开发了用于非人灵长类动物全脑记录的1,024通道高密度探针。相关研究成果2024年6月24日在线发表于《自然—神经科学》杂志上。

据介绍，灵长类动物大脑中单细胞分辨率的大规模神经群体记录仍然具有挑战性。

研究人员介绍了Neuroscroll探针，它可以同时从1024个密集分布的通道中分离出单个神经元活动，这些通道灵活地分布在探针的柄上。Neuroscroll探针的长度可以很容易地对10毫米到90毫米的单个探针进行调整，覆盖非人灵长类动物和人类的大脑大小，并且探针在反复弯曲变形后仍然完好无损。Neuroscroll探针能可靠地记录大鼠体内大量神经群的数据，其长期稳定性高达105周。每个Neuroscroll探针的记录同时从分布在恒河猴大脑整个深度的多个大脑区域产生了数百个分离良好的单个单元。凭借数千个同时记录的通道、前所未有的探针长度、优异的机械稳定性和灵活的记录位点分布，

总之，这一研究表明，Neuroscroll探针在系统神经科学研究中实现了广泛的新实验范式，具有很大的通用性。

附：英文原文

Title: A high-density 1,024-channel probe for brain-wide recordings in non-human primates

Author: Liu, Yang, Jia, Huilin, Sun, Hongji, Jia, Shengyi, Yang, Ziqian, Li, Ao, Jiang, Anqi, Naya, Yuji, Yang, Cen, Xue, Shengyuan, Li, Xiaojian, Chen, Bingyan, Zhu, Jingjun, Zhou, Chenghao, Li, Minning, Duan, Xiaojie

Issue&Volume: 2024-06-24

Abstract: Large-scale neural population recordings with single-cell resolution across the primate brain remain challenging. Here we introduce the Neuroscroll probe that isolates single neuronal activities simultaneously from 1,024 densely spaced channels that are flexibly distributed across the shank of the probe. The Neuroscroll probe length is easily tunable for individual probes from 10mm to 90mm, covering the brain size of non-human primates and humans, and the probes remain intact and functional after repeated bending deformations. The Neuroscroll probes provided reliable recordings from large neural populations with high chronic stability up to 105weeks in rats. Recording with each Neuroscroll probe yielded hundreds of well-isolated single units simultaneously from multiple brain regions distributed across the entire depth of the rhesus macaque brain. With the thousand simultaneously recorded channels, unprecedented probe length, excellent mechanical stability and flexible recording site distribution, the Neuroscroll probes enable a wide range of new experimental paradigms in system neuroscience studies with great versatility.

DOI: 10.1038/s41593-024-01692-6

Source: https://www.nature.com/articles/s41593-024-01692-6