近日，以色列内盖夫本古里安大学的Alberto Bilenca及其研究团队取得一项新进展。经过不懈努力，他们研制出布里渊增益显微镜。相关研究成果已于2024年6月10日在国际知名学术期刊《自然—光子学》上发表。

该研究团队开发了布里渊增益显微镜，可以检测与样品中感兴趣的布里渊声学振动模式相对应的特定机械对比频率的布里渊增益。与受激布里渊散射显微镜相比，布里渊增益显微镜的时间分辨率提高了200倍，在激发能低至23μJ时分辨率可达100μs。使用布里渊增益显微镜，研究人员成功展示了生命系统中材料的横截面，实现了全光学机械成像，并深入探讨了其结构和动力学特性。

据悉，相较于传统的机械成像方法，具有机械对比度的光学成像技术，如被动微流变学、光学相干弹性成像和布里渊显微镜，以其低微扰特性在材料和生物发现中扮演着关键角色。其中，受激布里渊散射显微镜作为一种新兴的光学显微镜技术，在生物力学成像中展现出了高灵敏度和特异性，已被证实极具价值。然而，其激发能量较高，且由于全光谱的获取需求，时间分辨率仍受到一定限制。

附：英文原文

Title: Brillouin gain microscopy

Author: Shaashoua, Roni, Kasuker, Lir, Kishner, Mor, Levy, Tal, Rotblat, Barak, Ben-Zvi, Anat, Bilenca, Alberto

Issue&Volume: 2024-06-10

Abstract: Optical imaging techniques with mechanical contrast, including passive microrheology, optical coherence elastography and Brillouin microscopy, are critical for material and biological discovery owing to their less perturbative nature compared with traditional mechanical imaging methods. An emerging optical microscopy approach for mechanical imaging is stimulated Brillouin scattering microscopy, which has been shown to be useful for biomechanical imaging with high sensitivity and specificity. However, the excitation energy used is high and the temporal resolution remains limited by the need to acquire full spectra. Here we develop Brillouin gain microscopy that detects the Brillouin gain at a specific mechanically contrasting frequency corresponding to a Brillouin acoustic-vibrational mode of interest in the sample. Brillouin gain microscopy affords a 200-fold improvement in temporal resolution compared with stimulated Brillouin scattering microscopy, down to 100μs at excitation energy as low as 23μJ. Using Brillouin gain microscopy, we demonstrate cross-sectional, all-optical mechanical imaging of materials as well as of the structure and dynamics in living systems with low excitation energy and at high temporal resolution.

DOI: 10.1038/s41566-024-01445-8

Source: https://www.nature.com/articles/s41566-024-01445-8