加拿大多伦多大学Brenda J. Andrews和Charles Boone课题组取得一项新突破。他们提出了真核细胞周期中蛋白质组尺度运动和隔室连通性。这一研究成果于2024年3月6日发表在国际顶尖学术期刊《细胞》上。
通过对数百万活酵母细胞的图像应用两种不同的卷积神经网络，研究组解决了细胞周期中蛋白质组水平的浓度和定位动态，分辨率约为20个亚细胞定位类别。研究团队发现四分之一的蛋白质组显示细胞周期性，这些蛋白质往往在定位或浓度水平上受到控制，但不是同时受到控制。
不同水平的蛋白质调控优先用于细胞周期的不同方面，蛋白质浓度的变化主要参与细胞周期控制，蛋白质定位的变化主要参与细胞周期程序的生物物理实现。研究小组提供了一个资源，用于探索细胞周期中全局蛋白质组动态，这将有助于在系统水平上理解基本的生物学过程。
据了解，细胞周期的进展依赖于蛋白质组的组成和亚细胞定位的协调变化。
Title: Proteome-scale movements and compartment connectivity during the eukaryotic cell cycle
Author: Athanasios Litsios, Benjamin T. Grys, Oren Z. Kraus, Helena Friesen, Catherine Ross, Myra Paz David Masinas, Duncan T. Forster, Mary T. Couvillion, Stefanie Timmermann, Maximilian Billmann, Chad Myers, Nils Johnsson, L. Stirling Churchman, Charles Boone, Brenda J. Andrews
Issue&Volume: 2024-03-06
Abstract: Cell cycle progression relies on coordinated changes in the composition and subcellular localization of the proteome. By applying two distinct convolutional neural networks on images of millions of live yeast cells, we resolved proteome-level dynamics in both concentration and localization during the cell cycle, with resolution of ～20 subcellular localization classes. We show that a quarter of the proteome displays cell cycle periodicity, with proteins tending to be controlled either at the level of localization or concentration, but not both. Distinct levels of protein regulation are preferentially utilized for different aspects of the cell cycle, with changes in protein concentration being mostly involved in cell cycle control and changes in protein localization in the biophysical implementation of the cell cycle program. We present a resource for exploring global proteome dynamics during the cell cycle, which will aid in understanding a fundamental biological process at a systems level.
DOI: 10.1016/j.cell.2024.02.014
Source: https://www.cell.com/cell/fulltext/S0092-8674(24)00180-6